Birnbaum M J, Kelly T J, Woods C W, Imberski R B
Gen Comp Endocrinol. 1984 Oct;56(1):9-18. doi: 10.1016/0016-6480(84)90055-8.
The effects of juvenile hormone (JH) and egg development neurosecretory hormone (EDNH) on ovarian ecdysteroid production during vitellogenesis in the autogenous mosquito, Aedes atropalpus, were investigated using in vitro techniques coupled with radioimmunoassay (RIA) and high-pressure liquid chromatography (HPLC). Normal females were characterized by quantitative, qualitative, and temporal patterns of in vitro ovarian ecdysteroid production. Females decapitated at emergence showed little ovarian ecdysteroid production and did not undergo vitellogenesis. A 500-ng dose of JH-I applied topically to decapitated females restored normal patterns of ecdysteroid production. In both normal and experimental females, ecdysone constituted the major portion of the ecdysteroids secreted by the ovaries in vitro. However, significant amounts of other RIA-active materials were detected, one of which was probably 20-OH-ecdysone. Fat body incubations indicated that these tissues produce little RIA-active material during the peak of vitellogenesis. During that period, the ovaries were the major source of ecdysteroid. Various doses of JH-I, applied to abdomens isolated at emergence, enhanced ovarian responsiveness to subsequent applications of head extracts containing EDNH both in vivo and in vitro. A 500-ng dose of JH-I, applied topically to blood-fed, decapitated Aedes aegypti, stimulated a significant increase in in vitro ovarian ecdysteroid production. Similarities between these data and those demonstrating prothoracicotropic effects of JH in the Lepidoptera are discussed.
采用体外技术结合放射免疫测定法（RIA）和高压液相色谱法（HPLC），研究了保幼激素（JH）和卵发育神经分泌激素（EDNH）对自育性蚊虫——阿氏伊蚊卵黄发生期卵巢蜕皮甾体生成的影响。正常雌蚊的体外卵巢蜕皮甾体生成具有定量、定性和时间模式特征。羽化时断头的雌蚊卵巢蜕皮甾体生成极少，且未发生卵黄发生。对断头雌蚊局部施用500 ng剂量的JH-I可恢复蜕皮甾体生成的正常模式。在正常和实验雌蚊中，蜕皮酮是卵巢体外分泌的蜕皮甾体的主要成分。然而，检测到大量其他具有RIA活性的物质，其中一种可能是20-羟基蜕皮酮。脂肪体孵育表明，在卵黄发生高峰期，这些组织产生的具有RIA活性的物质很少。在此期间，卵巢是蜕皮甾体的主要来源。对羽化时分离的腹部施用不同剂量的JH-I，可增强卵巢在体内和体外对随后施用的含EDNH头部提取物的反应性。对血餐后断头的埃及伊蚊局部施用500 ng剂量的JH-I，可刺激体外卵巢蜕皮甾体生成显著增加。讨论了这些数据与那些证明JH对鳞翅目昆虫具有促前胸腺作用的数据之间的相似性。
