Matsunaga Yoshiko, Koda Masahiko, Murawaki Yoshikazu
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago 683-8504, Japan.
Res Commun Mol Pathol Pharmacol. 2004;115-116:143-50.
Matrix metalloproteinases (MMPs) have been implicated in the tumor invasion and growth through the degradation of extracellular matrix. In this study, we selected 46 hepatocellular carcinoma (HCC) cases, at random, and we immunohistologically examined the expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, tissue inhibitor of metalloproteinases (TIMP)-1, TIMP-2, in cancerous and non-cancerous areas using avidin-biotin-peroxidase complex method. In all cases, cancer cells, hepatocytes, sinusoidal lining cells, leukocytes, and bile ducts were positive for all the primary antibodies. The expressions of MMPs and TIMPs in most of the HCC tissues were equal or low compared with those in the surrounding non-tumor tissues, although mixed expression pattern were recognized in some HCC tissues. The difference of MMP and TIMP expression was not related with the histological differentiation of HCC and the condition of non-cancerous area. These findings suggested little association of the clinicopathological findings of HCC with the histological expression of MMPs and TIMPs.
基质金属蛋白酶（MMPs）通过降解细胞外基质参与肿瘤侵袭和生长。在本研究中，我们随机选取了46例肝细胞癌（HCC）病例，采用抗生物素蛋白-生物素-过氧化物酶复合物法，对癌组织和非癌组织中MMP-1、MMP-2、MMP-3、MMP-7、MMP-9、金属蛋白酶组织抑制剂（TIMP）-1、TIMP-2的表达进行了免疫组织化学检测。在所有病例中，癌细胞、肝细胞、窦状隙衬里细胞、白细胞和胆管对所有一抗均呈阳性。与周围非肿瘤组织相比，大多数HCC组织中MMPs和TIMPs的表达相等或较低，尽管在一些HCC组织中观察到混合表达模式。MMP和TIMP表达的差异与HCC的组织学分化及非癌区域的情况无关。这些发现提示HCC的临床病理表现与MMPs和TIMPs的组织学表达之间几乎没有关联。
