Poulsen K, Nielsen A H, Johannessen A
Institute for Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Can J Physiol Pharmacol. 1991 Sep;69(9):1381-4. doi: 10.1139/y91-205.
In a new method for measurement of inactive rat plasma renin, the trypsin generated angiotensin I immunoreactive material, which was HPLC characterized as similar to tetradecapeptide renin substrate, is removed by a cation exchange resin before the renin incubation step. The method also corrects for trypsin destruction of endogenous angiotensinogen by the addition of exogenous angiotensinogen. When measured with this method inactive renin in rat plasma decreased after nephrectomy and increased after adrenalectomy. This is in accordance with findings in humans. A sexual dimorphism of prorenin (inactive renin) in rat plasma, similar to that reported in humans and mice, was demonstrated. Thus, inactive renin in the rat is no exception among species, and the rat might be a suitable animal model for further studies dealing with the physiology of prorenin in plasma and tissues.
在一种测量无活性大鼠血浆肾素的新方法中，胰蛋白酶产生的血管紧张素I免疫反应性物质（经高效液相色谱法鉴定与十四肽肾素底物相似）在肾素孵育步骤之前通过阳离子交换树脂去除。该方法还通过添加外源性血管紧张素原校正了内源性血管紧张素原被胰蛋白酶破坏的情况。用这种方法测量时，大鼠血浆中的无活性肾素在肾切除术后降低，在肾上腺切除术后升高。这与人类的研究结果一致。大鼠血浆中前肾素（无活性肾素）的性别二态性得到了证实，类似于在人类和小鼠中报道的情况。因此，大鼠体内的无活性肾素在物种中并无例外，大鼠可能是进一步研究血浆和组织中前肾素生理学的合适动物模型。
