Evans D B, Cornette J C, Sharma S K
Upjohn Company, Kalamazoo, MI 49001.
Clin Exp Hypertens A. 1990;12(8):1473-87. doi: 10.3109/10641969009073531.
We have studied the effects of bilateral nephrectomy and adrenalectomy on angiotensinogen concentration, plasma renin activity, and total plasma renin activity obtained after trypsin activation in rats and compared with controls. Our results show that the plasma angiotensinogen concentration of nephrectomized (NX) rats is 5-fold higher than that of adrenalectomized (AX) rats. On the other hand, plasma angiotensinogen concentration of AX rats was about 2.5-fold lower than that of control rats. While NX rat plasma possess no renin activity, its mixing (1:1, v/v) with AX plasma results in 2-fold increase in renin activity over that observed for AX plasma alone. These results suggest that the apparent increase in renin activity upon mixing these two plasmas is at least partly due to an overall increase in angiotensinogen concentration in the mixture. To show that it is not due to activation of NX plasma prorenin by a convertase from AX plasma, prorenin-free NX rat plasma was prepared by using an anti-renin immunoaffinity column. When this prorenin-free NX plasma was mixed (1:1, v/v) with AX, again a 2-fold increase in renin activity was observed which is attributed to the overall increase in plasma angiotensinogen in the mixture. It is concluded that rat plasma prorenin is probably not activated within the circulation by a prorenin convertase from the rat kidney.
我们研究了双侧肾切除和肾上腺切除对大鼠血管紧张素原浓度、血浆肾素活性以及胰蛋白酶激活后获得的总血浆肾素活性的影响，并与对照组进行了比较。我们的结果表明，肾切除（NX）大鼠的血浆血管紧张素原浓度比肾上腺切除（AX）大鼠高5倍。另一方面，AX大鼠的血浆血管紧张素原浓度比对照大鼠低约2.5倍。虽然NX大鼠血浆没有肾素活性，但将其与AX血浆以1:1（v/v）混合后，肾素活性比单独观察到的AX血浆增加了2倍。这些结果表明，混合这两种血浆后肾素活性的明显增加至少部分是由于混合物中血管紧张素原浓度的总体增加。为了表明这不是由于AX血浆中的转化酶激活了NX血浆中的肾素原，我们使用抗肾素免疫亲和柱制备了无肾素原的NX大鼠血浆。当这种无肾素原的NX血浆与AX以1:1（v/v）混合时，再次观察到肾素活性增加了2倍，这归因于混合物中血浆血管紧张素原的总体增加。得出的结论是，大鼠血浆肾素原可能不会在循环中被大鼠肾脏中的肾素原转化酶激活。
