Wang Mei-Fei, Lian Hong-Zhen, Mao Li, Zhou Jing-Ping, Gong Hui-Juan, Qian Bao-Yong, Fang Yan, Li Jie
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China), School of Chemistry and Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu Province 210093, China.
J Agric Food Chem. 2007 Jul 11;55(14):5552-8. doi: 10.1021/jf063623q. Epub 2007 Jun 12.
A capillary gas chromatographic (GC) method has been developed for the separation and determination of policosanol components extracted from rice bran wax. A Varian CP-sil 8 CB column was employed, and an oven temperature was programmed. Gas chromatography-mass spectrometry (GC-MS) was used to identify the composition of policosanol. Quantitative analysis was carried out by means of hydrogen flame ionization detector (FID) with dinonyl phthalate (DNP) as internal standard. The results indicated that the extract obtained by dry saponification has the highest contents of octacosanol and triacontanol among extracts by all used extraction methods including dry saponification, saponification in alcohol, saponification in water (neutralized and non-neutralized), and transesterification. Meanwhile, the GC-MS fingerprint of policosanol extracted by dry saponification has been established. Euclidean distance similarity calculation showed remarkable consistency of compositions and contents among 12 batches of policosanol from a rice bran wax variety. This protocol provided a rapid and feasible method for quality control of policosanol products.
建立了一种毛细管气相色谱（GC）法，用于分离和测定从米糠蜡中提取的多元醇成分。采用Varian CP-sil 8 CB柱，并对柱温进行程序升温。利用气相色谱-质谱联用（GC-MS）对多元醇的组成进行鉴定。采用氢火焰离子化检测器（FID），以邻苯二甲酸二壬酯（DNP）为内标进行定量分析。结果表明，在所采用的所有提取方法（包括干皂化法、醇中皂化法、水中皂化法（中和及未中和）和酯交换法）中，干皂化法得到的提取物中二十八烷醇和三十烷醇的含量最高。同时，建立了干皂化法提取的多元醇的GC-MS指纹图谱。欧氏距离相似度计算表明，来自同一米糠蜡品种的12批多元醇在组成和含量上具有显著一致性。该方法为多元醇产品的质量控制提供了一种快速可行的方法。
