Comeau André M, Suttle Curtis A
Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4.
Environ Microbiol. 2007 Jul;9(7):1790-800. doi: 10.1111/j.1462-2920.2007.01299.x.
This study examined the distribution, susceptibility to viral infection and genetic diversity of Vibrio spp. in the coastal waters and sediments of British Columbia during summer (July and August). Abundances of presumptive Vibrio spp. ranged from 1.5 to 346 ml(-1) within the water column (1-291 m); whereas, abundances at the water-sediment interface were much higher (up to approximately 3 x 10(4)Vibrio spp. cc(-1)), and decreased with sediment depth (down to 30 cm). The genetic diversity of Vibrio spp. isolates was not tied to the location from which they originated and was only influenced in a minor way by the type of environment. However, the environment had a greater effect on phage-typing patterns. Vibrio parahaemolyticus isolates from environments with high abundances of cells (sediments and oysters) were generally more susceptible to viral infection than those from the water column which were highly resistant. Therefore, although Vibrio spp. were widespread in the areas investigated, the results show that there is segregation of bacterial host strains in different environments, under differing selection pressures, which ultimately will affect in situ phage production.
本研究调查了夏季（7月和8月）不列颠哥伦比亚沿海水域和沉积物中弧菌属的分布、对病毒感染的敏感性及遗传多样性。假定弧菌属在水柱（1 - 291米）中的丰度范围为1.5至346个/毫升；而在水 - 沉积物界面处的丰度要高得多（高达约3×10⁴个弧菌属/立方厘米），并随沉积物深度（至30厘米）而降低。弧菌属分离株的遗传多样性与它们的来源地无关，仅受到环境类型的轻微影响。然而，环境对噬菌体分型模式有更大影响。来自细胞丰度高的环境（沉积物和牡蛎）的副溶血性弧菌分离株通常比来自水柱的分离株更易受到病毒感染，而水柱中的分离株具有高度抗性。因此，尽管弧菌属在所调查区域广泛存在，但结果表明，在不同的选择压力下，不同环境中存在细菌宿主菌株的分离，这最终将影响原位噬菌体的产生。
