Meng Xue, Wang Min, You Siyuan, Wang Duobing, Li Yan, Liu Zhaoyang, Gao Yu, Liu Lu, Zhang Yaoyuan, Yan Zhenghao, Liu Chunyan, Jiang Yong, Shao Hongbing
College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
Curr Microbiol. 2017 Jul;74(7):815-820. doi: 10.1007/s00284-017-1221-2. Epub 2017 Apr 20.
A novel Siphoviridae family Phage BS5, which infects Pseudoalteromonas atlantica, was isolated from the surface waters of the Yellow Sea. Morphological study by transmission electron microscopy revealed that the novel phage belongs to Siphoviridae. The complete genome sequence of PBS5 contained a linear, double-strand 39949-bp DNA molecule with a G + C content of 40.6% and 65 putative open reading frames. Twelve conserved domains were detected by BLASTP in NCBI, and of these the functions of 5 were known. The genome was grouped into four modules as follows: phage structure, phage packaging, DNA replication and regulation, and some additional functions.16 S rDNA sequence analysis was also applied to identify the host bacteria. After initial characterization of bacteriophage PBS5, it was found that the optimal pH was 7.0, the optimal temperature was 30 °C, and the burst size was about 95 virions per cell. This information will provide an important benchmark for further research on the interaction between bacteriophages and their hosts.
从黄海表层水体中分离出一种新型的感染大西洋假交替单胞菌的长尾噬菌体科噬菌体BS5。通过透射电子显微镜进行的形态学研究表明，这种新型噬菌体属于长尾噬菌体科。噬菌体PBS5的完整基因组序列包含一个线性双链39949 bp的DNA分子，G + C含量为40.6%，有65个推定的开放阅读框。通过NCBI中的BLASTP检测到12个保守结构域，其中5个的功能已知。基因组分为以下四个模块：噬菌体结构、噬菌体包装、DNA复制与调控以及一些其他功能。还应用16S rDNA序列分析来鉴定宿主细菌。在对噬菌体PBS5进行初步表征后，发现其最适pH为7.0，最适温度为30℃，裂解量约为每个细胞95个病毒粒子。这些信息将为进一步研究噬菌体与其宿主之间的相互作用提供重要的基准。
