Hu Zhiping, Zeng Liuwang, Xie Lesi, Lu Wei, Zhang Jie, Li Ting, Wang Xiang
Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Neurochem Res. 2007 Nov;32(11):1927-31. doi: 10.1007/s11064-007-9382-1. Epub 2007 Jun 13.
Golgi apparatus (GA) is a very important organelle involved in the metabolism of numerous proteins. TGF-beta1 plays an important role in supporting neuronal survival after ischemic insults. Little is known, however, about the morphological alteration of GA and subcellular compartmentalization of TGF-beta1 in brain after ischemia. Therefore, our present study was designed to check for GA morphological alterations and TGF-beta1 subcellular localization. GA immunoreactivities were examined in the somatosensory cortex of gerbils after 10 min transient forebrain ischemia. Confocal Immunofluorographs of TGF-beta1 and TGN38 were also taken. Results indicated that no fragmentation of GA was found in gerbils of norm, shams and 6, 24 and 72 h postocclusion, but some of the cortical cells showed fragmentation of GA in gerbils 7 days postocclusion. TGF-beta1 was colocalized with TGN38, a marker molecule for the GA. We conclude that there was morphological alterations of GA and TGF-beta1 was present in GA in the somatosensory cortex after 10 min ischemia.
高尔基体（GA）是一种非常重要的细胞器，参与多种蛋白质的代谢。转化生长因子-β1（TGF-β1）在缺血性损伤后支持神经元存活方面发挥着重要作用。然而，关于缺血后脑内高尔基体的形态改变以及TGF-β1的亚细胞定位，人们了解甚少。因此，我们目前的研究旨在检查高尔基体的形态改变以及TGF-β1的亚细胞定位。在沙土鼠短暂性前脑缺血10分钟后，检测其体感皮层中的高尔基体免疫反应性。还拍摄了TGF-β1和TGN38的共聚焦免疫荧光照片。结果表明，在正常沙土鼠、假手术组以及闭塞后6小时、24小时和72小时的沙土鼠中，未发现高尔基体断裂，但在闭塞后7天的沙土鼠中，一些皮层细胞出现了高尔基体断裂。TGF-β1与高尔基体的标记分子TGN38共定位。我们得出结论，在缺血10分钟后，体感皮层中的高尔基体存在形态改变，且TGF-β1存在于高尔基体中。
