Yuan Yasheng, Chi Fanglu, Wang Shuyi, Wang Zhengmin
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.
ORL J Otorhinolaryngol Relat Spec. 2007;69(5):283-8. doi: 10.1159/000103872. Epub 2007 Jun 12.
To investigate the expression of ceramide produced by sphingomyelin and DNA ploidy in normal laryngeal mucosa, laryngeal precancerous lesions and laryngeal carcinoma.
Eighty consecutive patients with leukoplakia larynx were identified from the archived pathology files of the Eye, Ear, Nose and Throat Hospital of Fudan University from 1990 to 2001. Among them, 23 patients developed laryngeal carcinoma. Flow cytometry and immunohistochemistry were performed to test DNA content and ceramide expression on normal tissue, precancerous lesions and laryngeal carcinoma.
Among 80 patients with laryngeal carcinoma, 23 cases were all aneuploids, and 57 diploids. Expression of ceramide decreased gradually from normal tissue via precancerous lesions to laryngeal carcinoma. Cell staining per high-power field was 400 +/- 30, 180 +/- 20, and 10 +/- 10, respectively (p < 0.05, t test). The expression of ceramide in the DNA diploid cell was higher than that in the aneuploid cell (p < 0.05, t test).
Ceramide, the second messenger in apoptosis, plays a significant role in the progression from precancerous lesion to carcinoma of the larynx. Reduction in ceramide may be the key factor in laryngeal carcinogenesis.
探讨鞘磷脂产生的神经酰胺在正常喉黏膜、喉癌前病变及喉癌中的表达及DNA倍体情况。
从复旦大学附属眼耳鼻喉科医院1990年至2001年的存档病理文件中选取80例连续性喉白斑患者。其中，23例发展为喉癌。采用流式细胞术和免疫组化法检测正常组织、癌前病变及喉癌的DNA含量和神经酰胺表达。
80例喉癌患者中，23例为非整倍体，57例为二倍体。神经酰胺的表达从正常组织经癌前病变至喉癌逐渐降低。每高倍视野细胞染色分别为400±30、180±20和10±10（p<0.05，t检验）。神经酰胺在DNA二倍体细胞中的表达高于非整倍体细胞（p<0.05，t检验）。
神经酰胺作为凋亡的第二信使，在喉癌前病变进展为癌的过程中起重要作用。神经酰胺减少可能是喉癌发生的关键因素。
