Bernard A, Lauwerys R, Noel A, Vandeleene B, Lambert A
Unité de Toxicologie Industrielle et de Médecine du Travail, Université Catholique de Louvain, Brussels, Belgium.
Clin Chim Acta. 1991 Sep 30;201(3):231-45. doi: 10.1016/0009-8981(91)90374-l.
A sensitive latex particle assay has been developed to study the occurrence of protein 1 in human urine. The coefficients of variation (CVs) of the method which is fully automated vary between 3 and 11.5%. The assayable concentration range is 0.3 to 40 micrograms/l. Protein 1 is clearly a sex-dependent protein. In contrast to urinary retinol-binding protein (RBP) which shows no variation with age or sex, protein 1 is excreted in greater amounts in males from the puberty. In adults, the mean concentration of protein 1 in urine of men is approximately 5 times that of women. In the urine from both sexes, protein 1 occurs as a single component with a Mr around 21,000 and an pI of about 4.8. Protein 1 is correlated with RBP in the urine from female or male patients with impaired proximal tubular function, which suggests that it is handled by the kidney in a similar manner as RBP. Diabetics, however, show elevations of urinary protein 1 which do not correlate with the RBP excretion but with the albuminuria. A competition between albumin and protein 1 for renal tubular uptake might explain this paradoxical behaviour of protein 1 in the course of diabetic nephropathy.
已开发出一种灵敏的乳胶颗粒分析法来研究人尿中蛋白质1的存在情况。这种全自动方法的变异系数（CVs）在3%至11.5%之间。可检测的浓度范围是0.3至40微克/升。蛋白质1显然是一种性别依赖性蛋白质。与尿视黄醇结合蛋白（RBP）不同，RBP的排泄量不随年龄或性别而变化，蛋白质1从青春期开始在男性中排泄量更大。在成年人中，男性尿中蛋白质1的平均浓度约为女性的5倍。在两性的尿液中，蛋白质1以单一成分形式存在，其相对分子质量约为21,000，等电点约为4.8。在近端肾小管功能受损的女性或男性患者的尿液中，蛋白质1与RBP相关，这表明它在肾脏中的处理方式与RBP相似。然而，糖尿病患者尿中蛋白质1升高，这与RBP排泄量无关，而与蛋白尿有关。白蛋白和蛋白质1在肾小管摄取方面的竞争可能解释了蛋白质1在糖尿病肾病过程中的这种矛盾行为。
