Nomori H, Morinaga S, Kobayashi R, Torikata C
Department of Surgery, Saiseikai Central Hospital, Tokyo, Japan.
Virchows Arch. 1994;424(5):517-23. doi: 10.1007/BF00191438.
Thirty-six different normal tissues and 13 different malignant epithelial tumours, were examined immunohistochemically for the presence of protein 1 (P1) and Clara cell 10-kDa protein (CC10). Adenocarcinomas of the lung were also examined for the expression of pulmonary surfactant apoprotein using a monoclonal antibody (PE-10). The staining results of P1 and CC10 were almost identical both in normal tissues and in malignant tumours. In normal lung, Clara cells were strongly positive for both P1 and CC10. In addition, some goblet cells and non-ciliated non-mucus cells in the upper airways were moderately positive for both proteins. In the malignant tumours, some lung cancers were positive for P1 and CC10, both of which were positive in the same tumour cells on sequential sections. In 117 lung cancers, P1 and CC10 were positive in 10.2% of adenocarcinomas, 20.5% of squamous cell carcinomas, and 12.5% of large cell carcinomas. PE-10 stained positively in 65.3% of adenocarcinomas, a frequency significantly higher than that of P1 and CC10 (P < 0.01). These results suggest that P1 and CC10 are nearly identical proteins, that both are useful markers of Clara cells, and that many pulmonary adenocarcinomas express surfactant apoprotein rather than Clara cell proteins.
对36种不同的正常组织和13种不同的恶性上皮肿瘤进行免疫组织化学检查，以检测蛋白1（P1）和克拉拉细胞10 kDa蛋白（CC10）的存在情况。还使用单克隆抗体（PE-10）对肺腺癌进行肺表面活性物质载脂蛋白表达的检测。P1和CC10在正常组织和恶性肿瘤中的染色结果几乎相同。在正常肺组织中，克拉拉细胞对P1和CC10均呈强阳性。此外，上呼吸道的一些杯状细胞和无纤毛无黏液细胞对这两种蛋白均呈中度阳性。在恶性肿瘤中，一些肺癌对P1和CC10呈阳性，在连续切片的同一肿瘤细胞中两者均为阳性。在117例肺癌中，P1和CC10在10.2%的腺癌、20.5%的鳞状细胞癌和12.5%的大细胞癌中呈阳性。PE-10在65.3%的腺癌中呈阳性染色，其频率显著高于P1和CC10（P<0.01）。这些结果表明，P1和CC10是几乎相同的蛋白，两者都是克拉拉细胞的有用标志物，并且许多肺腺癌表达表面活性物质载脂蛋白而非克拉拉细胞蛋白。
