Dettmeyer Reinhard, Sperhake Jan P, Müller Jutta, Madea Burkhard
Department of Forensic Medicine, University of Bonn, Stiftsplatz 12, D-53111 Bonn, Germany.
Forensic Sci Int. 2008 Jan 30;174(2-3):229-33. doi: 10.1016/j.forsciint.2007.05.009. Epub 2007 Jun 13.
Immunohistochemical and molecularpathologic techniques have improved the diagnosis of myocarditis as compared with conventional histologic staining methods done according to the Dallas criteria. Additionally, immunohistochemistry and in situ-hybridization are able to demonstrate viral infection, e.g. cytomegaloviruses in salivary glands and lungs, locations both known to be involved in cytomegalovirusinfection. However, in many cases of proved cytomegalovirusinfection the cause of death remains unclear. We report on three children younger than 1-year of age, who died suddenly without prodromal symptoms. Their deaths were attributed to SIDS (sudden infant death syndrome). In situ-hybridization, immunohistochemical (LCA, CD45R0, CD68, MHC-class-II-molecules, E-selectine) and molecularpathologic investigations (PCR), however, suggested that death was caused by a cytomegalovirus-induced pneumonia or myocarditis. In the future, these methods should be used for investigating cases with suspicion of SIDS.
与根据达拉斯标准进行的传统组织学染色方法相比，免疫组织化学和分子病理学技术改善了心肌炎的诊断。此外，免疫组织化学和原位杂交能够证明病毒感染，例如唾液腺和肺部的巨细胞病毒感染，这两个部位都是已知参与巨细胞病毒感染的部位。然而，在许多已证实的巨细胞病毒感染病例中，死亡原因仍不清楚。我们报告了三名1岁以下的儿童，他们没有前驱症状突然死亡。他们的死亡被归因于婴儿猝死综合征（SIDS）。然而，原位杂交、免疫组织化学（LCA、CD45R0、CD68、MHC-II类分子、E-选择素）和分子病理学研究（PCR）表明，死亡是由巨细胞病毒引起的肺炎或心肌炎所致。未来，这些方法应用于调查疑似婴儿猝死综合征的病例。
