Kovács Gábor M, Balázs Tímea, Pénzes Zsolt
Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117,, Budapest, Hungary.
Institute of Genetics, Biological Research Center of Hungarian Academy of Sciences, P.O. Box 521, 6701,, Szeged, Hungary.
Mycorrhiza. 2007 Oct;17(7):597-605. doi: 10.1007/s00572-007-0137-2. Epub 2007 Jun 14.
The arbuscular mycorrhizal (AM) fungi colonizing the sporophytes of the eusporangiate rattlesnake fern (Botrychium virginianum, Ophioglossaceae) in its Hungarian population were investigated in the present study. Different regions of the nrRNA gene complex were analyzed using two different primer sets. These produced similar results for the detected AM fungi phylotypes. Several AM fungal lineages were associated with sporophytes of B. virginianum. Phylogenetic analyses of different partial small subunit datasets grouped one lineage into the Gigasporaceae, showing similarities with Scutellospora sequences. In addition to unidentified Scutellospora phylotypes, it is possible that S. gregaria also colonized the fern. Several AM fungal phylotypes colonizing the sporophytes grouped into Glomus group A. They did not form distinct clades but grouped with sequences of AM fungi with different geographic and host origins. One main lineage clustered into the widespread G. fasciculatum/G. intraradices group and one into the subgroup GlGrAc, while others had no affinity to the subgroups of Glomus group A. As AM fungal phylotypes associated with B. virginianum seem to belong to widespread AM fungal taxa and show no specificity to this fern, we suppose that the previously described special anatomy of AM of B. virginianum is determined by the plant.
本研究调查了匈牙利种群中定殖于厚囊蕨类植物弗吉尼亚瓶尔小草（Botrychium virginianum，瓶尔小草科）孢子体上的丛枝菌根（AM）真菌。使用两组不同的引物对nrRNA基因复合体的不同区域进行了分析。对于检测到的AM真菌系统型，这两组引物产生了相似的结果。几个AM真菌谱系与弗吉尼亚瓶尔小草的孢子体相关联。对不同部分小亚基数据集的系统发育分析将一个谱系归入巨孢囊霉科，显示出与盾巨孢囊霉序列的相似性。除了未鉴定的盾巨孢囊霉系统型外， gregaria盾巨孢囊霉也有可能定殖于该蕨类植物。定殖于孢子体的几个AM真菌系统型归入球囊霉属A组。它们没有形成明显的分支，而是与来自不同地理区域和宿主的AM真菌序列聚在一起。一个主要谱系聚入广泛分布的G. fasciculatum/G. intraradices组，一个聚入亚组GlGrAc，而其他谱系与球囊霉属A组的亚组没有亲缘关系。由于与弗吉尼亚瓶尔小草相关联的AM真菌系统型似乎属于广泛分布的AM真菌类群，且对该蕨类植物没有特异性，我们推测先前描述的弗吉尼亚瓶尔小草AM的特殊解剖结构是由植物决定的。
