亚分子层的实时定量成像

Real-time quantitative imaging of submolecular layers.

作者信息

Ausserré Dominique, Khachfe Refahi Abou

机构信息

Laboratoire de Physique de l'Etat Condensé (UMR 6087), Université du Maine, CNRS, Route de Laval, 72000 Le Mans, France.

出版信息

Langmuir. 2007 Jul 17;23(15):8015-20. doi: 10.1021/la0636098. Epub 2007 Jun 14.

DOI:10.1021/la0636098

PMID:17567158

Abstract

Using a recent optical contrast method, real-time and quantitative imaging of submolecular layers was performed with the help of a simple optical microscope. The measuring technique is exposed and documented by three examples. In particular, it allowed label-free detection of peptide-antibody binding interactions with 50 pg/mm2 sensitivity while keeping full optical lateral resolution.

摘要

使用一种最新的光学对比方法，借助一台简单的光学显微镜对亚分子层进行了实时定量成像。通过三个实例展示并记录了该测量技术。特别地，它能够以50 pg/mm²的灵敏度对肽 - 抗体结合相互作用进行无标记检测，同时保持完整的光学横向分辨率。