Owens Róisín M, Wang ChangQing, You Juyoung A, Jiambutr Jantorn, Xu Arron S L, Marala Ravi B, Jin Moonsoo M
Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, USA.
J Recept Signal Transduct Res. 2009;29(3-4):195-201. doi: 10.1080/10799890903079919.
Real-time detection of viral replication inside cells remains a challenge to researchers. The Epic System is a high-throughput, label-free optical detection platform capable of measuring molecular interaction in a biochemical assay, as well as integrated cellular response from measurement of cellular dynamic mass redistribution (DMR) in a cell-based assay. DMR has previously been used to measure cell signaling upon receptor stimulation. In this report, we present the first example of Epic measurement of viral replication-induced cellular response and demonstrate that this system is extremely powerful not only for the sensitive and quantitative detection of viral replication inside cells but also for screening of viral inhibitors. By comparing with conventional assays used for the measurement of viral replication, we show that the Epic response has many advantages including sensitivity, high throughput, real-time quantification and label-free detection. We propose that the Epic system for measurement of integrated cellular response will be an excellent method for elucidating steps in viral replication as well as for the high-throughput screening of inhibitors of rhinovirus and other viruses.
实时检测细胞内的病毒复制对研究人员来说仍然是一项挑战。Epic系统是一个高通量、无标记的光学检测平台，能够在生化分析中测量分子相互作用，以及在基于细胞的分析中通过测量细胞动态质量重分布（DMR）来测量综合细胞反应。DMR此前已被用于测量受体刺激后的细胞信号传导。在本报告中，我们展示了Epic测量病毒复制诱导的细胞反应的首个实例，并证明该系统不仅对于细胞内病毒复制的灵敏和定量检测极为强大，而且对于病毒抑制剂的筛选也非常有效。通过与用于测量病毒复制的传统分析方法进行比较，我们表明Epic反应具有许多优点，包括灵敏度、高通量、实时定量和无标记检测。我们提出，用于测量综合细胞反应的Epic系统将是阐明病毒复制步骤以及高通量筛选鼻病毒和其他病毒抑制剂的优秀方法。
