Jia Xiaohong, Ekman Maria, Grove Harald, Faergestad Ellen M, Aass Laila, Hildrum Kjell I, Hollung Kristin
Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.
J Proteome Res. 2007 Jul;6(7):2720-31. doi: 10.1021/pr070173o. Epub 2007 Jun 14.
Postmortem changes in protein composition up to 24 h in bovine longissimus thoracis muscle were investigated by two-dimensional gel electrophoresis and MALDI-TOF MS/MS. A total of 47 spots were significantly changed the first 24 h postmortem. The 39 identified proteins can be divided into five groups: metabolic enzymes, defense and stress proteins, structural proteins, proteolytic enzymes, and unclassified proteins. The identified metabolic enzymes are all associated with ATP-generating pathways, either the glycolytic pathway or energy metabolism. In addition, several defense and stress proteins were changed in abundance in this study. These findings contribute to a better understanding of the biochemical processes during postmortem storage of meat.
通过二维凝胶电泳和基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF MS/MS）研究了牛胸最长肌死后24小时内蛋白质组成的变化。死后最初24小时内共有47个斑点发生了显著变化。鉴定出的39种蛋白质可分为五组：代谢酶、防御和应激蛋白、结构蛋白、蛋白水解酶和未分类蛋白。鉴定出的代谢酶均与ATP生成途径有关，即糖酵解途径或能量代谢。此外，本研究中几种防御和应激蛋白的丰度发生了变化。这些发现有助于更好地理解肉类死后储存期间的生化过程。
