Jia Xiaohong, Hollung Kristin, Therkildsen Margrethe, Hildrum Kjell Ivar, Bendixen Emøke
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.
Proteomics. 2006 Feb;6(3):936-44. doi: 10.1002/pmic.200500249.
To study early post-mortem changes in muscle tissues from bull calves, cytosole proteins from two muscles: M. longissimus dorsi (LD) and M. semitendinosis (ST) at 0 and 24 h after slaughter were analysed by 2-DE. Principal component analysis (PCA) and rotation testing were used to analyse the protein patterns in the two muscles in order to select protein spots that were significantly different at the two time-points. Selected proteins were identified by MALDI-TOF/TOF. Five proteins, namely cofilin, lactoylglutathione lyase, substrate protein of mitochondrial ATP-dependent proteinase SP-22, HSP 27 and HSP20, were changed in both LD and ST muscles during post-mortem storage. Fifteen additional protein changes were observed in either LD or ST muscles, and some of these changes have not previously been observed to change during post-mortem storage of bovine muscles. Further studies will reveal the relevance of these biomarkers for meat quality.
为研究公牛犊牛肌肉组织的早期死后变化，采用双向电泳（2-DE）分析了屠宰后0小时和24小时两块肌肉（背最长肌（LD）和半腱肌（ST））的胞质蛋白。运用主成分分析（PCA）和旋转测试来分析两块肌肉中的蛋白质图谱，以选择在两个时间点有显著差异的蛋白质斑点。通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF）鉴定所选蛋白质。在死后储存期间，LD和ST肌肉中共有五种蛋白质发生了变化，分别是肌动蛋白结合蛋白、乳酰谷胱甘肽裂解酶、线粒体ATP依赖性蛋白酶SP-22的底物蛋白、热休克蛋白27（HSP 27）和热休克蛋白20（HSP20）。在LD或ST肌肉中还观察到另外15种蛋白质的变化，其中一些变化在牛肌肉死后储存期间以前未被观察到。进一步的研究将揭示这些生物标志物与肉质的相关性。
