Janulaityte-Günther Daiva, Kupcinskas Limas, Pavilonis Alvydas, Valuckas Konstantinas, Wadström Torkel, Andersen Leif Percival
Department of Microbiology, University of Medicine, Kaunas, Lithuania.
FEMS Immunol Med Microbiol. 2007 Jul;50(2):220-5. doi: 10.1111/j.1574-695X.2007.00268.x.
Helicobacter pylori is a major factor for the development of gastric cancer. The aim of this study was to define serum antibody patterns associated with H. pylori infection in patients with gastric cancer using a Western blot technique. Serum samples collected from 115 patients with gastric cancer and 110 age- and gender-matched patients without gastrointestinal diseases were tested for IgG antibodies to H. pylori antigens (outer membrane proteins and whole cell preparations). No significant differences were found between patients with and without gastric cancer using outer membrane proteins (82% and 73%, P>0.05) or whole cell antigens (84% and 76%, P>0.05), respectively. The significant differences between patients with and without gastric cancer were associated with bands of 94 kDa (54% and 20%, P<0.001) and 30 kDa (65% and 44%, P<0.01). A combination of antibodies to 85 kDa (VacA) and 120 kDa (CagA) was significantly (P<0.01) more frequent in gastric cancer patients than in patients without gastric cancer. The detection of antibodies to 94- and 30-kDa bands, in association with the determination of serum antibodies to CagA+/VacA+, may have a prospective value in assessment of the risk of developing of gastric cancer.
幽门螺杆菌是胃癌发生的主要因素。本研究的目的是使用蛋白质印迹技术确定胃癌患者中与幽门螺杆菌感染相关的血清抗体模式。对115例胃癌患者以及110例年龄和性别匹配的无胃肠道疾病患者采集的血清样本检测幽门螺杆菌抗原（外膜蛋白和全菌制剂）的IgG抗体。分别使用外膜蛋白（82%和73%，P>0.05）或全菌抗原（84%和76%，P>0.05）时，胃癌患者与非胃癌患者之间未发现显著差异。胃癌患者与非胃癌患者之间的显著差异与94 kDa条带（54%和20%，P<0.001）和30 kDa条带（65%和44%，P<0.01）有关。与无胃癌患者相比，胃癌患者中85 kDa（VacA）和120 kDa（CagA）抗体的组合明显更常见（P<0.01）。检测94 kDa和30 kDa条带的抗体，结合检测CagA+/VacA+血清抗体，可能对评估胃癌发生风险具有前瞻性价值。
