Choi Seong-Jun, Na Kun, Kim Sungwon, Woo Dae Gyun, Sun Bo Kyung, Chung Hyung-Min, Park Keun-Hong
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok2-dong, Wonmi-gu, Bucheon, 420-743, Korea.
J Biomed Mater Res A. 2007 Dec 15;83(4):897-905. doi: 10.1002/jbm.a.31403.
This study evaluated the potential of using poly(NiPAAm-co-AAc) as an injectable drug delivery carrier and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. In particular, rabbit chondrocytes were embedded in hydrogels containing a combination of ascorbate and transforming growth factor beta-3 (TGF beta-3). Hydrogel constructs containing embedded cells either without ascorbate or a combination of ascorbate and TGF beta-3 were used as controls to determine the effects of ascorbate and TGF beta-3 on chondrogenic differentiation. The level of cartilage associated ECM proteins was examined using immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. The results showed that ascorbate is an important factor for preparing cartilage constructs because of its action on chondrocyte phenotype modulation and proliferation.
本研究评估了聚（N-异丙基丙烯酰胺-共-丙烯酸）作为可注射药物递送载体和细胞治疗剂的潜力，其以支持基质的形式用于兔软骨细胞的软骨形成分化。具体而言，将兔软骨细胞包埋在含有抗坏血酸和转化生长因子β-3（TGF-β3）组合的水凝胶中。使用不含抗坏血酸或抗坏血酸与TGF-β3组合的包埋细胞的水凝胶构建体作为对照，以确定抗坏血酸和TGF-β3对软骨形成分化的影响。使用II型胶原的免疫组织化学染色以及番红O和阿尔辛蓝（糖胺聚糖）染色来检查软骨相关细胞外基质蛋白的水平。结果表明，抗坏血酸因其对软骨细胞表型调节和增殖的作用，是制备软骨构建体的重要因素。
