Na Kun, Kim Sungwon, Woo Dae-Gyun, Sun Bo Kyung, Yang Han Na, Chung Hyung-Min, Park Keun-Hong
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok 2-dong, Wonmi-gu, Bucheon 420-743, Republic of Korea.
J Biotechnol. 2007 Feb 1;128(2):412-22. doi: 10.1016/j.jbiotec.2006.09.025. Epub 2006 Oct 12.
In this study, a hydrogel composite, based on the thermo-reversible hydrogel of p(NiPAAm-co-AAc) and hyaluronic acid (HA) was used as an injectable cell and growth factor carrier for cartilage tissue engineering applications. Rabbit chondrocytes were embedded in blended hydrogel composites co-encapsulated with the transforming growth factor beta-3 (TGFbeta-3). The blended hydrogel with the embedded chondrocytes and HA co-encapsulating unloaded growth factors and those with the thermo-reversible hydrogel were used as the controls to examine the effects of TGFbeta-3 on neocartilage formation. The blended hydrogel loaded with TGFbeta-3 embedded with chondrocytes were injected subcutaneously into the nude mice. The mice were monitored for 8 weeks after the injection. Both the differentiation and level of cartilage-specific ECM production were significantly higher in the presence of HA and growth factor than in the control without the growth factor. The level of cartilage associated ECM proteins was examined by immunohistochemical staining (collagen types II and X) as well as by Safranin-O and Alcian blue (GAG) staining. The results showed the potential application of blended hydrogel mixed with the growth factor to neocartilage formation.
在本研究中，一种基于聚（N-异丙基丙烯酰胺-共-丙烯酸）（p(NiPAAm-co-AAc)）热可逆水凝胶和透明质酸（HA）的水凝胶复合材料被用作软骨组织工程应用中的可注射细胞和生长因子载体。兔软骨细胞被包埋在与转化生长因子β-3（TGFβ-3）共包封的混合水凝胶复合材料中。将包埋有软骨细胞且HA共包封空载生长因子的混合水凝胶以及含有热可逆水凝胶的混合水凝胶用作对照，以研究TGFβ-3对新软骨形成的影响。将负载有TGFβ-3且包埋有软骨细胞的混合水凝胶皮下注射到裸鼠体内。注射后对小鼠进行8周监测。与不含生长因子的对照组相比，在存在HA和生长因子的情况下，软骨特异性细胞外基质（ECM）产生的分化和水平均显著更高。通过免疫组织化学染色（II型和X型胶原）以及番红O和阿尔新蓝（糖胺聚糖，GAG）染色来检测软骨相关ECM蛋白的水平。结果表明，混合有生长因子的水凝胶在新软骨形成方面具有潜在应用价值。
