2-脱氧-2-氟-D-半乳糖蛋白N-糖基化

2-Deoxy-2-fluoro-D-galactose protein N-glycosylation.

作者信息

Loch N, Geilen C C, Spörndle I, Oberdorfer F, Keppler D, Tauber R, Reutter W

机构信息

Institut für Molekularbiologie und Biochemie Freien Universität Berlin, Germany.

出版信息

FEBS Lett. 1991 Dec 9;294(3):217-20. doi: 10.1016/0014-5793(91)81433-9.

DOI:10.1016/0014-5793(91)81433-9

PMID:1756864

Abstract

2-Deoxy-2-fluoro-D-galactose (dGalF), added to the medium of primary cultured rat hepatocytes, inhibited N-glycosylation of membrane (gp 120) and secretory glycoproteins (alpha 1-macroglobulin) in a concentration-dependent manner. Complete inhibition of N-glycosylation was achieved at concentrations of 1 mM and above. At identical concentrations, 2-deoxy-2-fluoro-D-glucose (dGlcF) caused only incomplete inhibition of N-glycosylation. dGalF reduced incorporation of D-[2,6-3H]mannose into lipid-linked oligosaccharides indicating interference with their assembly in the dolichol cycle.

摘要

添加到原代培养大鼠肝细胞培养基中的2-脱氧-2-氟-D-半乳糖（dGalF）以浓度依赖性方式抑制膜糖蛋白（gp120）和分泌性糖蛋白（α1-巨球蛋白）的N-糖基化。在1 mM及以上浓度时可完全抑制N-糖基化。在相同浓度下，2-脱氧-2-氟-D-葡萄糖（dGlcF）仅引起N-糖基化的不完全抑制。dGalF减少了D-[2,6-3H]甘露糖掺入脂质连接寡糖，表明其干扰了寡糖在多萜醇循环中的组装。