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塞姆利基森林病毒和流感病毒糖蛋白的体外糖基化及其被核苷酸 -2-脱氧己糖的抑制作用

Glycosylation in vitro of Semliki-Forest-virus and influenza-virus glycoproteins and its suppression by nucleotide-2-deoxy-hexose.

作者信息

Schwarz R T, Schmidt M F, Lehle L

出版信息

Eur J Biochem. 1978 Apr;85(1):163-72. doi: 10.1111/j.1432-1033.1978.tb12224.x.

DOI:10.1111/j.1432-1033.1978.tb12224.x
PMID:639813
Abstract

Cell-free enzyme preparations from cultured fibroblasts infected with Semliki forest virus or fowl plague virus (an influenza A virus) incorporate [14C]-mannose into dolichol-phosphate-mannose, lipid-linked oligosaccharides and into endogenous virus-specific glycoproteins. When GDP-2-deoxy-D-[14C]glucose serves as substrate 2-deoxy-D-[14C]glucose is transferred to dolichol phosphate yielding dolichol-monophosphate-2-deoxy-D-[14C]glucose. UDP-2-deoxy-D-[14C]glucose gives rise also to a lipid which, however, is not a polyprenol derivative. The transfer of [14C]mannose to lipid-extractable fractions and glycoproteins in vitro is blocked by GDP-2-deoxy-D-glucose. It can be restored by exogenous dolichol monophosphate only with regard to the formation of dolichol-monophosphate-[14C]mannose-labelled oligosaccharides into glycoproteins. UDP-2-deoxy-D-glucose has no inhibitory effect on transfer reactions of [14C]mannose from GDP-[14C]mannose into various lipid fractions or into glycoprotein. It is concluded therefore, that the inhibition of glycosylation brought about by 2-deoxyglucose in vivo is caused by an interference of its GDP derivative with the formation of a correct lipid-oligosaccharide.

摘要

用Semliki森林病毒或禽瘟病毒（一种甲型流感病毒）感染培养的成纤维细胞所制备的无细胞酶制剂，可将[14C]甘露糖掺入到多萜醇磷酸甘露糖、脂连接寡糖以及内源性病毒特异性糖蛋白中。当GDP-2-脱氧-D-[14C]葡萄糖作为底物时，2-脱氧-D-[14C]葡萄糖会转移至多萜醇磷酸，生成多萜醇单磷酸-2-脱氧-D-[14C]葡萄糖。UDP-2-脱氧-D-[14C]葡萄糖也会产生一种脂质，但它不是聚异戊二烯衍生物。体外实验中，GDP-2-脱氧-D-葡萄糖可阻断[14C]甘露糖向可提取脂质部分和糖蛋白的转移。只有在将多萜醇单磷酸-[14C]甘露糖标记的寡糖掺入糖蛋白的过程中，外源性多萜醇单磷酸才能恢复这种转移。UDP-2-脱氧-D-葡萄糖对[14C]甘露糖从GDP-[14C]甘露糖向各种脂质部分或糖蛋白的转移反应没有抑制作用。因此可以得出结论，体内2-脱氧葡萄糖所导致的糖基化抑制是由其GDP衍生物干扰正确的脂寡糖形成所致。

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引用本文的文献

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Wilehm Roux Arch Dev Biol. 1980 Feb;189(1):81-82. doi: 10.1007/BF00848571.
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