Iudici Neto Francesco, Pianetti-Filho Geraldo, Araújo Ricardo Nascimento, Nascimento Evaldo
Divisão de Epidemiologia e Controle de Doenças, Instituto Octávio Magalhães, Fundação Ezequiel Dias, Belo Horizonte, MG.
Rev Soc Bras Med Trop. 2007 Mar-Apr;40(2):163-9. doi: 10.1590/s0037-86822007000200004.
Crude antigen and semi-purified proteins from scolices of Taenia solium cysticerci were evaluated for the immunodiagnosis of human neurocysticercosis neurocysticercosis. Semi-purified proteins obtained by electrophoresis on polyacrylamide gel and by electroelution were tested by means of the immunoenzymatic reaction against sera from normal individuals and from patients with neurocysticercosis or other parasitic diseases. The 100 kDa protein provided 100% sensitivity and specificity in the immunodiagnosis. When 95 or 26 kDa proteins were used, 95 and 100% sensitivity and specificity were obtained, respectively. The assays involving crude antigen and sera from normal individuals or from patients with neurocysticercosis, diluted to 1:256, gave excellent agreement with those in which 100, 95 or 26 kDa proteins were tested against the same serum samples diluted to 1:64. (Kappa: 0.95 to 1.00). Crude scolex antigen may be useful for serological screening, while 100, 95 or 26 kDa protein can be used in confirmatory tests on neurocysticercosis-positive cases.
对来自猪带绦虫囊尾蚴头节的粗抗原和半纯化蛋白进行了人类神经囊尾蚴病免疫诊断评估。通过聚丙烯酰胺凝胶电泳和电洗脱获得的半纯化蛋白，采用免疫酶反应检测，以针对正常个体以及患有神经囊尾蚴病或其他寄生虫病患者的血清。100 kDa蛋白在免疫诊断中提供了100%的敏感性和特异性。当使用95 kDa或26 kDa蛋白时，分别获得了95%和100%的敏感性和特异性。涉及粗抗原以及稀释至1:256的正常个体或神经囊尾蚴病患者血清的检测，与针对稀释至1:64的相同血清样本检测100 kDa、95 kDa或26 kDa蛋白的检测结果高度一致（卡帕值：0.95至1.00）。粗头节抗原可用于血清学筛查，而100 kDa、95 kDa或26 kDa蛋白可用于对神经囊尾蚴病阳性病例的确诊检测。
