Suzuki Lisandra Akemi, Rossi Cláudio Lúcio
Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, SP, Brazil.
Arq Neuropsiquiatr. 2011 Jun;69(3):470-4. doi: 10.1590/s0004-282x2011000400012.
To evaluate the performance of two antigenic preparations (vesicular fluid - VF and a glycoprotein fraction, LLa-Gp fraction, purified from a whole parasite extract by lentil lectin affinity chromatography) from Taenia solium cysticerci for the immunodiagnosis of neurocysticercosis.
Fifty-six cerebrospinal fluid (CSF) samples (22 from patients with neurocysticercosis and 34 from patients with other neurological disorders) and 57 serum samples (22 from patients with neurocysticercosis, 18 from patients with other infections and 17 from presumably healthy persons) were assayed for anticysticercal IgG antibodies with an enzyme-linked immunosorbent assay (ELISA).
The VF ELISA showed 100% sensitivity and specificity in CSF and serum samples, whereas the sensitivity and specificity of the LLa-Gp ELISA were, respectively, 90.9% and 97.1%, with the CSF samples and 95.5% and 100% with serum samples. There was no significant difference in the sensitivity and specificity of the two antigenic preparations used to screen CSF and serum samples.
Considering the complexity and high cost of obtaining the LLa-Gp fraction, VF could be more suitable for screening specific antibodies by ELISA in CSF and serum samples from patients with neurocysticercosis.
评估猪带绦虫囊尾蚴的两种抗原制剂（囊泡液 - VF和通过扁豆凝集素亲和层析从整个寄生虫提取物中纯化得到的糖蛋白组分，LLa - Gp组分）用于神经囊尾蚴病免疫诊断的性能。
采用酶联免疫吸附测定（ELISA）检测56份脑脊液（CSF）样本（22份来自神经囊尾蚴病患者，34份来自其他神经系统疾病患者）和57份血清样本（22份来自神经囊尾蚴病患者，18份来自其他感染患者，17份来自推测为健康的个体）中的抗囊尾蚴IgG抗体。
VF - ELISA在CSF和血清样本中显示出100%的敏感性和特异性，而LLa - Gp - ELISA在CSF样本中的敏感性和特异性分别为90.9%和97.1%，在血清样本中分别为95.5%和100%。用于筛查CSF和血清样本的两种抗原制剂的敏感性和特异性没有显著差异。
考虑到获得LLa - Gp组分的复杂性和高成本，VF可能更适合通过ELISA在神经囊尾蚴病患者的CSF和血清样本中筛查特异性抗体。
