Maeda T, Sumida T, Kurasawa K, Tomioka H, Itoh I, Yoshida S, Koike T
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Diabetes. 1991 Dec;40(12):1580-5. doi: 10.2337/diab.40.12.1580.
This study analyzed T-lymphocyte-receptor V beta genes of infiltrating lymphocytes into the pancreases of 12- to 16-wk-old NOD mice with severe insulitis and 5-wk-old mice with mild insulitis by the quantitative polymerase chain reaction method. The V beta transcripts on infiltrating T lymphocytes into pancreases with severe insulitis in older NOD mice were diverse. In contrast, the V beta 11 gene transcript was predominantly expressed on T lymphocytes in the pancreas in younger NOD mice with mild insulitis, suggesting the possible role of V beta 11+ T lymphocytes in triggering insulitis in this species.
本研究采用定量聚合酶链反应方法，分析了12至16周龄患有严重胰岛炎的非肥胖糖尿病（NOD）小鼠以及5周龄患有轻度胰岛炎的NOD小鼠胰腺中浸润淋巴细胞的T淋巴细胞受体Vβ基因。在年龄较大的患有严重胰岛炎的NOD小鼠胰腺中，浸润性T淋巴细胞上的Vβ转录本具有多样性。相比之下，在年龄较小的患有轻度胰岛炎的NOD小鼠胰腺中，Vβ11基因转录本主要在T淋巴细胞上表达，这表明Vβ11 + T淋巴细胞在该物种引发胰岛炎中可能发挥作用。
