[胃康宁含药血清对胃癌细胞生长、血管内皮生长因子及其受体KDR和Flt-1表达的影响]

[Effects of weikangning contained serum on growth of gastric cancer cell, expression of vascular endothelial growth factor and its receptors including KDR and fit-1].

作者信息

Li Qing-ming, Kan Fang-ju, Min Cun-yun

机构信息

Department of Traditional Chinese Medicine, The Second Hospital Affiliated to Sun Yat-sen University, Guangzhou.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Jun;26 Suppl:88-92.

PMID:17569358

Abstract

OBJECTIVE

To observe the effect of drug-containing serum of Chinese traditional herbal decoction Weikangning (WKN) on growth of gastric cancer cell, expression of vascular endothelial growth factor (VEGF) and its receptors including KDR and Flt-1.

METHODS

A total of 120 male Wistar rats were given high, medium and low-dose WKN. After the drug-containing serum was prepared, the gastric cancer cells MGC-803 of different dose groups were cultured with the drug-containing serum, respectively. The gastric cell growth was observed by using light microscope and flow cytometer,the expression of VEGF and its receptor Flt-1 was detected with SABC immunohistochemistry method and the mRNA expression levels of VEGF and its receptors including KDR and Flt-1 of different groups were detected with reverse transcription-polymerase chain reaction (RT-PCR), respectively.

RESULTS

The gastric cancer cell growth and cell cycle of the three medicated groups were significantly improved as compared with those of the control group (P <0. 01) ,the proportion of cells in G0 - G1 phase was increased,while the cells in S phase were decreased. It was shown that the apoptotic rates were increased in the medicated groups in a dose-dependent manner. The gray scales ( microm(2) ) of VEGF and Flt-1 in high, medium and low dose groups was 182. 44 +/-0. 54,178. 65 +/-0. 56,174. 80 +/-0. 81 and 168. 51 +/- 0. 81,162. 01 +/-0. 52,148. 20 +/-0. 69, respectively vs 147.82 +/-0. 15(P <0.01) and 144.31 +/-0.71 (P <0.01) in the control group. The mRNA expression of VEGF and its receptors significantly decreased in gastric cancer cells after cultured with WKN contained serum (P < 0. 01 ).

CONCLUSION

WKN has inhibitory effects on gastric cancer cell growth and mRNA expression of VEGF as well as its receptors KDR and Flt-1.

摘要

目的

观察中药复方胃康宁（WKN）含药血清对胃癌细胞生长、血管内皮生长因子（VEGF）及其受体KDR和Flt-1表达的影响。

方法

将120只雄性Wistar大鼠分为高、中、低剂量WKN组，制备含药血清后，分别用不同剂量组的含药血清培养胃癌细胞MGC-803。用光镜和流式细胞仪观察胃细胞生长情况，用SABC免疫组化法检测VEGF及其受体Flt-1的表达，用逆转录-聚合酶链反应（RT-PCR）分别检测不同组VEGF及其受体KDR和Flt-1的mRNA表达水平。

结果

与对照组相比，三个用药组的胃癌细胞生长和细胞周期均有显著改善（P<0.01），G0-G1期细胞比例增加，S期细胞减少。结果表明，用药组的凋亡率呈剂量依赖性增加。高、中、低剂量组VEGF和Flt-1的灰度值（μm²）分别为182.44±0.54、178.65±0.56、174.80±0.81和168.51±0.81、162.01±0.52、148.20±0.69，对照组分别为147.82±0.15（P<0.01）和144.31±0.71（P<0.01）。用含WKN血清培养后，胃癌细胞中VEGF及其受体的mRNA表达显著降低（P<0.01）。