[胃康宁汤对细胞周期蛋白E、细胞周期蛋白依赖性激酶2及p27表达的影响]
[Effect of weikangning decoction on expression of cyclin E, CDK2, and p27].
作者信息
Kan Fang-Ju, Li Qing-Ming, Zhong Wa, Liang Wei-Wen
机构信息
Department of Traditional Chinese Medicine of the Second Affiliated Hospital, Sun Yet-sen University, Guangzhou 510120, China.
出版信息
Zhong Yao Cai. 2006 Apr;29(4):341-5.
OBJECTIVE
To observe the effect of containing drugs serum of weikanguing decoction (WKN) on expression of Cyclin E, CDK2 (Cyclin dependant kinase 2, CDK2) and p27 in gastric cancer cell line MGC-803.
METHODS
Total of 120 male Wistar rats were divided into control group, high dose group, medium dose group and low dose group fed with natural saline, 20,10, and 5 g/kg of WKN, respectively. The experimental animals were finally killed for the preparation of drug-containing serum. The gastric cancer cell MGC-803 was cultured with the drug-containing serum drawn from the rats in different groups. The expression of Cyclin E, CDK2 and p27 was detected with immunohistochemistry method-SABC. The expression of mRNA of Cyclin E, CDK2 and p27 were detected with RT-PCR.
RESULTS
In high, medium and low dose groups, the gray scales of cyclin E, CDK2 and the OPDTI values of p27 increased significantly, but the gray scales of p27 and the OPDTI values of cyclin E, CDK2 decreased remarkble, compared with that in control group.
CONCLUSION
The decoction of WKN can decrease the expression of Cyclin E, CDK2, increases the expression of p27. This effect may be involved in mechanism of gastric cancer cell growth inhibition induced by WKN.
目的
观察胃康宁汤含药血清对胃癌细胞株MGC - 803中细胞周期蛋白E（Cyclin E）、细胞周期蛋白依赖性激酶2（Cyclin dependant kinase 2，CDK2）及p27表达的影响。
方法
将120只雄性Wistar大鼠分为对照组、高剂量组、中剂量组和低剂量组，分别给予生理盐水、20、10、5 g/kg胃康宁汤灌胃。最后处死实验动物制备含药血清。用不同组大鼠提取的含药血清培养胃癌细胞MGC - 803。采用免疫组织化学SABC法检测Cyclin E、CDK2及p27的表达。采用RT - PCR法检测Cyclin E、CDK2及p27的mRNA表达。
结果
与对照组比较，高、中、低剂量组中cyclin E、CDK2灰度值及p27的光密度积分值（OPDTI）显著升高，而p27灰度值及cyclin E、CDK2的OPDTI显著降低。
结论
胃康宁汤可降低Cyclin E、CDK2表达，增加p27表达。此作用可能参与胃康宁汤诱导胃癌细胞生长抑制的机制。
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