Yang Pingfang, Chen Hui, Liang Yu, Shen Shihua
Key laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Proteomics. 2007 Jul;7(14):2459-68. doi: 10.1002/pmic.200600215.
Two-week-old dark-grown rice seedlings were de-etiolated upon exposure to light. A comparison of 2-DE protein profiles between the dark-grown control and the rice seedlings illuminated respectively for 6, 12 and 24 h revealed 52 differentially expressed CBB-stained spots. Of these changed spots, the identity of 51 protein spots was determined by MALDI-TOF MS. Of these identified proteins, 13 proteins were related to light reactions of photosynthesis, photosynthetic carbon assimilation and chlorophyll biosynthesis, indicating the complex process of biogenesis of photosynthetic apparatus was correlated to the transition from a dark-grown (etiolated) to a light-grown (de-etiolated) morphology. In addition, three proteins related to antioxidation and detoxification decreased in de-etiolated rice seedlings implied, that the etiolated rice seedlings possibly be under an oxidative stress which could be released during their early stages of de-etiolation. The increase of S-adenosylmethionine synthetase that is involved in the biosynthesis of the phytohormone ethylene might contribute to the phenotypic development of the apical hook in the de-etiolated rice seedlings. These results yield a comprehensive picture of the post-transcriptional response for de-etiolation of rice seedlings and serve as a basic platform for further characterization of gene function and regulation in light-induced development of plants.
两周大的黑暗中生长的水稻幼苗在光照下进行脱黄化。对黑暗生长的对照以及分别光照6小时、12小时和24小时的水稻幼苗的双向电泳蛋白质图谱进行比较，发现有52个考马斯亮蓝染色斑点差异表达。在这些变化的斑点中，通过基质辅助激光解吸电离飞行时间质谱确定了51个蛋白质斑点的身份。在这些已鉴定的蛋白质中，有13种蛋白质与光合作用的光反应、光合碳同化和叶绿素生物合成有关，表明光合器官生物发生的复杂过程与从黑暗生长（黄化）到光照生长（脱黄化）形态的转变相关。此外，脱黄化水稻幼苗中三种与抗氧化和解毒相关的蛋白质减少，这意味着黄化水稻幼苗可能处于氧化应激状态，而这种应激在脱黄化早期可能会解除。参与植物激素乙烯生物合成的S-腺苷甲硫氨酸合成酶的增加可能有助于脱黄化水稻幼苗顶端弯钩的表型发育。这些结果全面描绘了水稻幼苗脱黄化的转录后反应情况，并为进一步表征植物光诱导发育中的基因功能和调控提供了一个基础平台。
