Department of Applied Biological Chemistzry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
J Proteome Res. 2012 Jan 1;11(1):331-47. doi: 10.1021/pr200852q. Epub 2011 Nov 11.
Illumination-induced greening in dark-grown plants is one of the most dramatic developmental processes known in plants. In our current study, we characterized the greening process of rice seedlings using comparative proteome analysis. We identified 886 different proteins in both whole cell lysates of illuminated and nonilluminated rice shoots and performed comparative proteome analysis based on the MS spectral intensities obtained for unique peptides from respective proteins. Furthermore, the changes in the levels of individual proteins were then compared with those of the corresponding mRNAs. The results revealed well-coordinated increases in the enzymes involved in the Calvin cycle at both the protein and mRNA levels during greening, and that the changes at the mRNA level precede those at the protein level. Although a much lower effect of illumination was found on the enzymes associated with glycolysis and the TCA cycle, coordinated increases during greening were evident for the enzymes involved in photorespiration and nitrogen assimilation as well as the components of the chloroplastic translational machinery. These results thus define the differential regulation of distinct biological systems during greening in rice and demonstrate the usefulness of comprehensive and comparative proteome analysis for the characterization of biological processes in plant cells.
暗培养植物的光诱导变绿是植物中已知的最显著的发育过程之一。在我们目前的研究中，我们使用比较蛋白质组分析来描述水稻幼苗的变绿过程。我们在光照和非光照的水稻芽的全细胞裂解物中分别鉴定了 886 种不同的蛋白质，并基于从各自蛋白质获得的独特肽的 MS 光谱强度进行了比较蛋白质组分析。此外，然后将个别蛋白质水平的变化与相应的 mRNA 水平进行比较。结果表明，在变绿过程中，卡尔文循环中的酶在蛋白质和 mRNA 水平上的协同增加，并且在蛋白质水平之前发生 mRNA 水平的变化。尽管光照对与糖酵解和三羧酸循环相关的酶的影响要小得多，但在变绿过程中，与光呼吸和氮同化以及叶绿体翻译机制的组成部分相关的酶的协同增加是明显的。这些结果因此定义了在水稻变绿过程中不同生物系统的差异调节，并证明了综合和比较蛋白质组分析在植物细胞中生物过程表征中的有用性。
