Perales Lorena, Peñarrubia Lola, Cornejo María-Jesús
Departmento de Biología Vegetal, Facultad de Biología, Avda. Dr Moliner 50, 46100 Burjasot, Valencia, Spain.
J Plant Physiol. 2008 Feb;165(2):159-71. doi: 10.1016/j.jplph.2006.12.012. Epub 2007 Jun 13.
The expression of the maize polyubiquitin gene promoter UBI1 in rice cells has been used to study the involvement of ubiquitin in cell protection responses to dehydration caused by osmotic, saline or freezing stress. The effect of these stresses on UBI1 activity was investigated by the use of stably transformed rice calli (UBI1:GUS), as well as by transient expression experiments performed with cell lines with high or low tolerance to each type of stress. The theoretical analysis of the UBI1 promoter shows several putative stress-regulated boxes that could account for the stress-related UBI1 induction pattern described in this work. We suggest that the study of the differential UBI1 promoter-driven expression in rice cell lines with different level of tolerance to stress might be useful to elucidate complex signal transduction pathways in response to dehydration stresses in monocots.
玉米多聚泛素基因启动子UBI1在水稻细胞中的表达已被用于研究泛素在细胞对渗透、盐或冷冻胁迫引起的脱水保护反应中的作用。通过使用稳定转化的水稻愈伤组织（UBI1:GUS），以及对每种胁迫具有高耐受性或低耐受性的细胞系进行瞬时表达实验，研究了这些胁迫对UBI1活性的影响。对UBI1启动子的理论分析显示了几个假定的胁迫调节框，这可以解释本研究中描述的与胁迫相关的UBI1诱导模式。我们认为，研究不同胁迫耐受性水平的水稻细胞系中UBI1启动子驱动的差异表达，可能有助于阐明单子叶植物对脱水胁迫响应的复杂信号转导途径。
