Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India.
Plant Cell Rep. 2012 Feb;31(2):271-9. doi: 10.1007/s00299-011-1161-4. Epub 2011 Oct 14.
The rice Ubiquitin1 (Ubi1) promoter was tested to evaluate its capacity to express the heterologous gusA gene encoding β-glucuronidase in transgenic rice tissue relative to the commonly used Ubi1 corn promoter and the rice gibberellic acid insensitive (GAI) gene promoter element. Experimental results showed increased expression of gusA gene in rice tissue when driven by the native Ubi1 promoter when compared to the use of corn Ubi1 promoter. Results further indicated that the cis-regulatory elements present in the native promoter element might have been responsible for high expression. However, the gusA gene expression level when driven by the rice GAI promoter was notably lower than both Ubi1 promoters. The present study, thus, for the first time helped to demonstrate that the native Ubi1 promoter is a promising genetic element in transgenic approaches for constitutive expression of any gene in rice tissue.
水稻泛素 1（Ubi1）启动子被测试用于评估其在转基因水稻组织中表达异源 gusA 基因（编码β-葡萄糖醛酸酶）的能力，相对于常用的玉米 Ubi1 启动子和水稻赤霉素不敏感（GAI）基因启动子元件。实验结果表明，与使用玉米 Ubi1 启动子相比，当由天然 Ubi1 启动子驱动时，gusA 基因在水稻组织中的表达增加。结果进一步表明，天然启动子元件中存在的顺式调控元件可能是高表达的原因。然而，由水稻 GAI 启动子驱动时，gusA 基因的表达水平明显低于两个 Ubi1 启动子。因此，本研究首次证明，天然 Ubi1 启动子是在转基因方法中在水稻组织中组成型表达任何基因的有前途的遗传元件。
