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九种人类胚胎干细胞系的LongSAGE分析

LongSAGE profiling of nine human embryonic stem cell lines.

作者信息

Hirst Martin, Delaney Allen, Rogers Sean A, Schnerch Angelique, Persaud Deryck R, O'Connor Michael D, Zeng Thomas, Moksa Michelle, Fichter Keith, Mah Diana, Go Anne, Morin Ryan D, Baross Agnes, Zhao Yongjun, Khattra Jaswinder, Prabhu Anna-Liisa, Pandoh Pawan, McDonald Helen, Asano Jennifer, Dhalla Noreen, Ma Kevin, Lee Stephanie, Ally Adrian, Chahal Neil, Menzies Stephanie, Siddiqui Asim, Holt Robert, Jones Steven, Gerhard Daniela S, Thomson James A, Eaves Connie J, Marra Marco A

机构信息

Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3.

出版信息

Genome Biol. 2007;8(6):R113. doi: 10.1186/gb-2007-8-6-r113.

DOI:10.1186/gb-2007-8-6-r113
PMID:17570852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2394759/
Abstract

To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.

摘要

为促进新型人类胚胎干细胞（ESC）转录本的发现，我们从9个人类胚胎干细胞系中生成了250万个长链分析基因表达标签（LongSAGE标签）。对这些数据的分析表明，与终末分化细胞相比，胚胎干细胞表达的RNA结合蛋白比例更高，并鉴定出了新型胚胎干细胞转录本，其中至少有一种可能代表多能状态的标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/e63d4141e4cc/gb-2007-8-6-r113-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/09e9f1bd4acf/gb-2007-8-6-r113-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/0fbe3e2ebac7/gb-2007-8-6-r113-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/232e479e21b3/gb-2007-8-6-r113-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/211653f84c87/gb-2007-8-6-r113-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/45ddf30a7c41/gb-2007-8-6-r113-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/e63d4141e4cc/gb-2007-8-6-r113-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/09e9f1bd4acf/gb-2007-8-6-r113-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/0fbe3e2ebac7/gb-2007-8-6-r113-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/232e479e21b3/gb-2007-8-6-r113-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/211653f84c87/gb-2007-8-6-r113-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/45ddf30a7c41/gb-2007-8-6-r113-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d951/2394759/e63d4141e4cc/gb-2007-8-6-r113-6.jpg

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本文引用的文献

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A mouse atlas of gene expression: large-scale digital gene-expression profiles from precisely defined developing C57BL/6J mouse tissues and cells.小鼠基因表达图谱：来自精确定义的发育中的C57BL/6J小鼠组织和细胞的大规模数字基因表达谱。
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Core transcriptional regulatory circuitry in human embryonic stem cells.人类胚胎干细胞中的核心转录调控回路。
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Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells.
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Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns.人类和小鼠胚胎干细胞和诱导多能干细胞中的 microRNA 谱分析揭示了重叠但不同的 microRNA 表达模式。
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Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells.成纤维细胞生长因子蛋白的热稳定性是调节人多能干细胞自我更新、分化和重编程的决定因素。
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Protein kinase C mediated extraembryonic endoderm differentiation of human embryonic stem cells.蛋白激酶 C 介导的人胚胎干细胞的胚外内胚层分化。
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Next-generation tag sequencing for cancer gene expression profiling.用于癌症基因表达谱分析的新一代标签测序
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MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells.人类胚胎干细胞分化过程中的微小RNA与基因表达模式
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Molecular profiling reveals similarities and differences between primitive subsets of hematopoietic cells generated in vitro from human embryonic stem cells and in vivo during embryogenesis.分子谱分析揭示了从人类胚胎干细胞体外生成的造血细胞原始亚群与胚胎发育过程中体内造血细胞原始亚群之间的异同。
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What would you do if you could sequence everything?如果你能对所有事物进行测序，你会怎么做？
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Transcribed processed pseudogenes in the human genome: an intermediate form of expressed retrosequence lacking protein-coding ability.人类基因组中转录加工的假基因：一种缺乏蛋白质编码能力的表达反转录序列的中间形式。
Nucleic Acids Res. 2005 Apr 28;33(8):2374-83. doi: 10.1093/nar/gki531. Print 2005.
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Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression.八聚体和Sox元件是Nanog基因表达转录顺式调控所必需的。
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Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001.2001年8月9日之前获得的三个人类胚胎干细胞系的核型稳定性、基因分型、分化、无饲养层培养及基因表达取样。
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Presence of functional gap junctions in human embryonic stem cells.人类胚胎干细胞中功能性缝隙连接的存在。
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Systematic recovery and analysis of full-ORF human cDNA clones.全长开放阅读框人源cDNA克隆的系统回收与分析
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