慢性阻塞性肺疾病（COPD）和哮喘患者血清及支气管灌洗液中白细胞介素-8（IL-8）、分泌性白细胞蛋白酶抑制因子（SLPI）、可溶性CD14（sCD14）和可溶性细胞间黏附分子-1（sICAM-1）的浓度

Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma.

作者信息

Hollander Camilla, Sitkauskiene Brigita, Sakalauskas Raimundas, Westin Ulla, Janciauskiene Sabina M

机构信息

Department of Otolaryngology and Head and Neck Surgery, University Hospital, 20502 Malmö, Sweden.

出版信息

Respir Med. 2007 Sep;101(9):1947-53. doi: 10.1016/j.rmed.2007.04.010. Epub 2007 Jun 15.

DOI:10.1016/j.rmed.2007.04.010

PMID:17574828

Abstract

BACKGROUND

Airway inflammation is associated with an increased expression and release of inflammatory reactants that regulate processes of cell migration, activation and degranulation. The purpose of this study was to quantify bronchial lavage (BAL) fluid and serum levels of chemokine (IL-8), secretory leukocyte protease inhibitor (SLPI), soluble intracellular adhesion molecules-1 (sICAM-1) and sCD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease (COPD) patients with similar disease duration time.

METHODS

Biomarkers in serum and BAL fluid from asthma (n=13) and COPD (n=25) patients were measured using commercially available ELISA kits.

RESULTS

We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of sICAM-1 and sCD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma (P<0.05). In both groups, BAL IL-8 correlated with SLPI (r=0.577, P<0.01 and r=0.589, P<0.05, respectively). In patients with COPD the BAL sICAM-1 correlated with sCD14 (r=0.576, P<0.01), while in asthma patients BAL sICAM-1 correlated with FEV(1)/FVC (r=0.418, P<0.01). Moreover, in asthma patients the serum SLPI correlated with sCD14 (r=0.688, P<0.01) and serum sICAM-1 negatively correlated with FEV(1)/FVC (r=-0.582, P<0.05).

CONCLUSION

Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma.

摘要

背景

气道炎症与炎症反应物的表达增加和释放有关，这些炎症反应物调节细胞迁移、激活和脱颗粒过程。本研究的目的是量化支气管灌洗（BAL）液和血清中趋化因子（IL-8）、分泌型白细胞蛋白酶抑制剂（SLPI）、可溶性细胞间黏附分子-1（sICAM-1）和sCD14的水平，作为疾病持续时间相似的哮喘和慢性阻塞性肺疾病（COPD）患者炎症和免疫反应的替代标志物。

方法

使用市售ELISA试剂盒检测哮喘患者（n = 13）和COPD患者（n = 25）血清和BAL液中的生物标志物。

结果

我们发现，在哮喘组和COPD组中，BAL液中IL-8和SLPI的浓度显著高于血清，而BAL液中sICAM-1和sCD14的水平显著低于血清。在这4种检测的生物标志物中，与哮喘患者相比，COPD患者中只有BAL液中的IL-8更高（P < 0.05）。在两组中，BAL液中的IL-8与SLPI相关（分别为r = 0.577，P < 0.01和r = 0.589，P < 0.05）。在COPD患者中BAL液中的sICAM-1与sCD14相关（r = 0.576，P < 0.01），而在哮喘患者中BAL液中的sICAM-1与FEV(1)/FVC相关（r = 0.418，P < 0.01）。此外，在哮喘患者中血清SLPI与sCD14相关（r = 0.688，P < 0.01），血清sICAM-1与FEV(1)/FVC呈负相关（r = -0.582，P < 0.05）。