Department of Biomaterials and Biotechnology, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV Groningen, The Netherlands.
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9713GZ Groningen, The Netherlands.
Nano Lett. 2024 Aug 7;24(31):9650-9657. doi: 10.1021/acs.nanolett.4c02409. Epub 2024 Jul 16.
Chronic obstructive pulmonary disease (COPD), the third leading cause of death worldwide, is caused by chronic exposure to toxic particles and gases, such as cigarette smoke. Free radicals, which are produced during a stress response to toxic particles, play a crucial role in disease progression. Measuring these radicals is difficult since the complex mixture of chemicals within cigarette smoke interferes with radical detection. We used a new quantum sensing technique called relaxometry to measure free radicals with nanoscale resolution on cells from COPD patients and healthy controls exposed to cigarette smoke extract (CSE) or control medium. Epithelial cells from COPD patients display a higher free radical load than those from healthy donors and are more vulnerable to CSE. We show that epithelial cells of COPD patients are more susceptible to the damaging effects of cigarette smoke, leading to increased release of free radicals.
慢性阻塞性肺疾病（COPD）是全球第三大致死原因，由长期接触有毒颗粒和气体（如香烟烟雾）引起。自由基在对有毒颗粒的应激反应中产生，在疾病进展中起着关键作用。由于香烟烟雾中化学物质的复杂混合物会干扰自由基的检测，因此测量这些自由基非常困难。我们使用一种称为弛豫测量法的新型量子传感技术，在 COPD 患者和健康对照者的细胞上测量自由基，这些细胞暴露于香烟烟雾提取物（CSE）或对照培养基中。来自 COPD 患者的上皮细胞显示出比来自健康供体的更高的自由基负荷，并且对 CSE 更敏感。我们表明，COPD 患者的上皮细胞更容易受到香烟烟雾的破坏作用，导致自由基释放增加。
