Webley G E, Richardson M C, Given A, Harper J
MRC/AFRC Comparative Physiology Group, Institute of Zoology, Regent's Park, London, UK.
Hum Reprod. 1991 Jul;6(6):779-82. doi: 10.1093/oxfordjournals.humrep.a137428.
Human granulosa cells, from women undergoing ovum collection for in-vitro fertilization (IVF), will luteinize in vitro and provide a model for investigating the antigonadotrophic action of a prostaglandin F2 alpha (PGF2 alpha) analogue, cloprostenol, on granulosa-derived luteal cells. The granulosa cells were cultured in a defined medium and exposed to treatments during a preincubation period of 0 to 3 days and a final incubation with low density lipoprotein (LDL) from days 3 to 4. In the absence of human chorionic gonadotrophin (HCG), progesterone production was low, whereas exposure to HCG in the final incubation resulted in a 10-fold increase in progesterone concentrations. The inclusion of cloprostenol with HCG in the final incubation significantly (P less than 0.05) inhibited HCG-stimulated progesterone production. Exposure to HCG during the preincubation prevented the antigonadotrophic action of cloprostenol in the final incubation. The antigonadotrophic action of cloprostenol was retained when the granulosa cells were exposed to cloprostenol during the preincubation. Omission of LDL from the final incubation lowered the production of progesterone but the pattern of responses to HCG and cloprostenol were similar. Prevention of the antigonadotrophic action of cloprostenol after exposure to HCG may be a mechanism through which chorionic gonadotrophin can prevent regression of the corpus luteum in early pregnancy. Cloprostenol does not appear to inhibit LDL-stimulated steroidogenesis in human granulosa cells.
从接受体外受精（IVF）取卵的女性身上获取的人颗粒细胞，会在体外发生黄体化，从而为研究前列腺素F2α（PGF2α）类似物氯前列醇对颗粒细胞来源的黄体细胞的抗促性腺激素作用提供一个模型。颗粒细胞在特定培养基中培养，并在0至3天的预孵育期接受处理，从第3天到第4天与低密度脂蛋白（LDL）进行最终孵育。在没有人绒毛膜促性腺激素（HCG）的情况下，孕酮分泌量很低，而在最终孵育中暴露于HCG会导致孕酮浓度增加10倍。在最终孵育中将氯前列醇与HCG一起使用可显著（P小于0.05）抑制HCG刺激的孕酮分泌。在预孵育期间暴露于HCG可防止氯前列醇在最终孵育中的抗促性腺激素作用。当颗粒细胞在预孵育期间暴露于氯前列醇时，氯前列醇的抗促性腺激素作用得以保留。在最终孵育中省略LDL会降低孕酮的分泌，但对HCG和氯前列醇的反应模式相似。在暴露于HCG后防止氯前列醇的抗促性腺激素作用可能是绒毛膜促性腺激素能够防止早孕时黄体退化的一种机制。氯前列醇似乎不会抑制人颗粒细胞中LDL刺激的类固醇生成。
