Yang Jun, Pi Xitian, Zhang Liguo, Liu Xiangshao, Yang Jing, Cao Yi, Zhang Wenxian, Zheng Xiaolin
Bioengineering College, Chongqing University, People's Republic of China.
Anal Sci. 2007 Jun;23(6):697-703. doi: 10.2116/analsci.23.697.
A series of symmetrical and asymmetrical microfluidic T-sensors with different inlet angles were fabricated to study the mixing characteristics of a T-type microstructure for generating concentration gradient. Computational fluid dynamics (CFD) simulations showed that the concentration gradient, transition zone and diffusion length were different for various configurations and inlet angles. Quick mix and sharp concentration gradient occurred in the asymmetrical structure with large inlet angle. The observed concentration gradients in the fabricated microchannel were consistent with the theoretical prediction. In this microstructure, stagnant zone and z-direction diffusion also affected the concentration gradient. Based on the simulation results, the microfluidic structure was optimized to generate desired concentration gradient for a cell-based study.
制备了一系列具有不同入口角度的对称和不对称微流控T型传感器，以研究用于产生浓度梯度的T型微结构的混合特性。计算流体动力学（CFD）模拟表明，不同结构和入口角度的浓度梯度、过渡区和扩散长度各不相同。在大入口角度的不对称结构中出现了快速混合和陡峭的浓度梯度。在制造的微通道中观察到的浓度梯度与理论预测一致。在这种微结构中，停滞区和z方向扩散也会影响浓度梯度。基于模拟结果，对微流控结构进行了优化，以产生用于细胞研究的所需浓度梯度。
