Homolka L, Lisá L, Kubatová A, Valqová M, Janderová B, Nerud F
Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Folia Microbiol (Praha). 2007;52(2):153-7. doi: 10.1007/BF02932154.
The viability, growth and morphology of 48 strains of Ascomycota (including 17 yeasts) and 20 strains of Zygomycota were determined after a 2-d and then after 1-year storage in liquid nitrogen using a new cryopreservation method with perlite as a particulate solid carrier. In case of Ascomycota, 45 strains (94 %) out of 48 survived both 2-d and 1-year storage in liquid nitrogen, respectively. In case of Zygomycota, all 20 strains survived both storage. In addition, 3 strains of Basidiomycota counted among yeasts were tested and all survived the 1 year storage. In all surviving cultures no negative effects of cryopreservation by this method have been observed after 1-year of storage in liquid nitrogen. The results indicate that the perlite protocol can be successfully used for cryopreservation of taxonomically different groups of fungi and also for fungi which failed to survive other routinely used preservation procedures.
使用以珍珠岩作为颗粒状固体载体的新型冷冻保存方法，在液氮中保存2天以及1年后，测定了48株子囊菌（包括17株酵母）和20株接合菌的活力、生长情况及形态。对于子囊菌，48株中有45株（94%）分别在液氮中保存2天和1年后存活。对于接合菌，所有20株均在两种保存条件下存活。此外，对3株属于酵母的担子菌进行了测试，所有菌株在保存1年后均存活。在液氮中保存1年后，在所有存活培养物中均未观察到该方法冷冻保存的负面影响。结果表明，珍珠岩方案可成功用于分类学上不同组别的真菌的冷冻保存，也可用于在其他常规保存程序中未能存活的真菌。
