Homolka Ladislav, Lisá Ludmila, Nerud Frantisek
Institute of Microbiology AS CR, Vídenská 1083, 142 20 Prague 4, Czech Republic.
J Microbiol Methods. 2007 Jun;69(3):529-32. doi: 10.1016/j.mimet.2006.09.004. Epub 2006 Oct 11.
Mycelial basidiomycete cultures on perlite in cryovials survived successfully three successive cycles of freezing, storage in liquid nitrogen (LN) and thawing without noticeable changes. This indicates that using perlite as a carrier for cryopreservation could in most cases overcome difficulties caused by interrupted supply of LN or electric power during the storage. Cultures on perlite can also be reused for successive inoculations.
保存在冻存管珍珠岩上的丝状担子菌培养物成功经受了三个连续的冷冻、液氮储存和解冻循环，且无明显变化。这表明，在大多数情况下，使用珍珠岩作为冷冻保存的载体可以克服储存期间液氮或电力供应中断所带来的困难。珍珠岩上的培养物还可重复用于后续接种。
