Wang Ming, Li Xiao-quan, Mo Zi-yao, Liu Yu-fei, Deng Zhi-ai, Zhang Xin-qiang, Shen Ji-chuan, Zhang Ying
Guangzhou Center for Disease Control and Prevention , Guangzhou 510080, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Jan;28(1):61-4.
To apply pulse-field gel electrophoresis analysis(PFGE) in the analysis of cholera outbreak events and to determine the molecular epidemiological characteristics of Vibrio cholerae ( V. cholerae) isolates.
PFGE using restriction enzyme Not I was employed in the molecular subtyping of forty-one strains of V. cholerae isolated in cholera outbreak events from 2003 to 2005 in Guangzhou area and PFGE patterns were analyzed by BioNumerics Version 4.0 software to perform cluster analysis. Pattern profiles were compared by utilizing of Dice coefficient and UPGMA(unweighted pair group method with arithmetic averages). Comparison of PFGE typing results was performed with phage-biological typing and pathogenicity-associated genes typing.
In cholera outbreak events, PFGE could discriminate epidemiologically related and unrelated strains, having more discriminatory power than phage-biological typing and pathogenicity-associated genes-typing.
Molecular sub-typing by PFGE could disclose the epidemiological relationships of strains from humans and the environment, providing molecular epidemiological evidence and support for the source-tracking of cholera outbreak events.
应用脉冲场凝胶电泳分析（PFGE）对霍乱暴发事件进行分析，并确定霍乱弧菌分离株的分子流行病学特征。
采用限制性内切酶Not I的PFGE技术对2003年至2005年广州地区霍乱暴发事件中分离的41株霍乱弧菌进行分子分型，并用BioNumerics 4.0软件分析PFGE图谱以进行聚类分析。利用Dice系数和非加权组平均法（UPGMA）比较图谱轮廓。将PFGE分型结果与噬菌体生物学分型和致病性相关基因分型进行比较。
在霍乱暴发事件中，PFGE能够区分流行病学相关和不相关的菌株，其鉴别力高于噬菌体生物学分型和致病性相关基因分型。
PFGE分子分型可揭示人与环境菌株的流行病学关系，为霍乱暴发事件的溯源提供分子流行病学证据和支持。
