1993年深圳地区霍乱弧菌分离株的脉冲场凝胶电泳分子分型研究
[Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993].
作者信息
Lan Quan-xue, Hu Qing-hua, Shi Xiao-lu, Wang Bing, Lin Yi-man, Zhang Shun-xiang, Cheng Jin-quan, Kan Biao, Xu Jian-guo
机构信息
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 May;28(5):491-4.
OBJECTIVE
To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.
METHODS
Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.
RESULTS
39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.
CONCLUSION
The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.
目的
确定1993年至2002年深圳不同霍乱弧菌分离株之间的遗传关系。
方法
用限制性内切酶Not I在海克姆金琼脂糖中消化60株分离株的染色体DNA，然后通过脉冲场凝胶电泳分析琼脂糖块。使用BioNumerics软件对霍乱弧菌分离株的脉冲场凝胶电泳（PFGE）图谱进行聚类分析。
结果
鉴定出39种独特的PFGE图谱，每种图谱有20至30条带。大多数PFGE图谱分为A簇或B簇。
结论
深圳确实存在密切相关的霍乱弧菌大流行克隆簇。霍乱弧菌的PFGE可用于霍乱的主动监测和追踪。
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