Jassim S A A, Griffiths M W
Department of Microbiology, Zayed Complex for Herbal Research and Traditional Medicine, General Authority for Health Services for the Emirate of Abu Dhabi, UAE.
Lett Appl Microbiol. 2007 Jun;44(6):673-8. doi: 10.1111/j.1472-765X.2007.02115.x.
To develop a method for rapid detection of bacteria via bacteriophage amplification coupled with exogenous fluorochromic stains.
A method for the rapid detection of bacteria was developed which consisted of exposing the sample suspected to contain target cells to host-specific phage. After at least one infection cycle, bacteria known to be infected by the phage (helper cells) were added and the number of nascent phage particles was estimated using the Live/Dead BacLight Bacterial Viability kit. Using Pseudomonas aeruginosa, it was shown that the dead helper cell population following phage infection was proportional to the initial number of target cells present in the original sample. Approximately 1 x 10(1) CFU per ml of P. aeruginosa could be detected within 4 h without the need for enrichment.
The phage lytic amplification assay coupled with exogenous fluorochromic stains was able to detect approx. 1 x 10(1) CFU per ml of the target bacterium within 4 h.
A method to detect low number of bacterial cells in a sample within 4 h without the need for enrichment was developed.
开发一种通过噬菌体扩增结合外源荧光染料快速检测细菌的方法。
开发了一种快速检测细菌的方法，该方法包括将疑似含有靶细胞的样品暴露于宿主特异性噬菌体。在至少一个感染周期后，加入已知被噬菌体感染的细菌（辅助细胞），并使用活/死细菌荧光试剂盒估计新生噬菌体颗粒的数量。以铜绿假单胞菌为例，结果表明噬菌体感染后死亡的辅助细胞数量与原始样品中存在的靶细胞初始数量成正比。无需富集，在4小时内可检测到每毫升约1×10¹CFU的铜绿假单胞菌。
噬菌体裂解扩增试验结合外源荧光染料能够在4小时内检测到每毫升约1×10¹CFU的靶细菌。
开发了一种无需富集即可在4小时内检测样品中少量细菌细胞的方法。
