Isolation of Cryptococcus neoformans from hollows of living trees in the city of Alfenas, MG, Brazil.

Cryptococcus neoformans is an encapsulated yeast, aetiological agent of cryptococcosis, commonly associated with pigeon droppings and plant materials. The species has also been associated with tree hollows. The aim of the present work was to verify the presence of the yeast in hollows of living trees and identify the isolates obtained in varieties and serotypes. Three samples were collected from 18 trees of five different species totalling 54 samples. Wood samples were collected by scraping the surface of the trunks and the inner face of the hollows. Samples were inoculated on to agar Niger medium for fungal isolation. The serotypes were determined by PCR using specific primers. Among the 54 samples evaluated, two were positive for the presence of C. n. var. neoformans (serotype A and MATalpha). The trees belonged to Caesalpinia peltophoroides and Anadenanthera peregrina species. The results of this study suggest that decayed wood obtained from hollows of C. peltophoroides and A. peregrina can be used as natural habitat for C. n. var. neoformans.