Lazéra M S, Cavalcanti M A, Trilles L, Nishikawa M M, Wanke B
Laboratório de Micologia Médica, Hospital Evandro Chagas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
Med Mycol. 1998 Apr;36(2):119-22.
To study hollows of living trees as the natural habitat of Cryptococcus neoformans in an endemic area of cryptococcosis in the northeastern Brazilian region, samples of decaying wood were collected inside the hollows, plated on niger seed agar and inoculated into mice and hamsters. Identification of C. neoformans was based on morphological and physiological tests. Canavanine-glycine-bromothymol medium was used to screen the varieties and Crypto Check Iatron Kit to serotype the isolates. For a period of 29 months C. neoformans var. gattii serotype B was isolated repeatedly from the hollow of a pottery tree (Moquilea tomentosa), pointing to the natural occurrence of C. neoformans var. gatti in decaying wood forming hollows in living trees. Evidence for a natural habitat of the variety gattii other than that related to Eucalyptus camaldulensis are discussed.
为了研究巴西东北部地区隐球菌病流行区活树树洞中作为新型隐球菌自然栖息地的情况，从树洞内采集腐朽木材样本，接种于 Niger 种子琼脂平板上，并接种到小鼠和仓鼠体内。新型隐球菌的鉴定基于形态学和生理学测试。使用刀豆氨酸-甘氨酸-溴百里酚蓝培养基筛选菌株变种，并使用 Crypto Check Iatron 试剂盒对分离株进行血清分型。在 29 个月的时间里，从一棵陶树（Moquilea tomentosa）的树洞中反复分离出新型隐球菌变种加蒂血清型 B，表明新型隐球菌变种加蒂在活树中形成树洞的腐朽木材中自然存在。文中讨论了除与赤桉相关的栖息地外，加蒂变种其他自然栖息地的证据。
