Saracli M A, Yildiran S T, Sener K, Gonlum A, Doganci L, Keller S M, Wickes B L
Division of Medical Mycology, Gulhane Military Medical Academy and School of Medicine, Etlik, Ankara, Turkey.
Mycoses. 2006 Mar;49(2):124-9. doi: 10.1111/j.1439-0507.2006.01203.x.
A total of 26 environmental Cryptococcus neoformans var. neoformans strains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed-field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating-type study, of 26 strains, 20 were MATalpha, four were MATa, one was MATa/alpha and one was non-typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATalpha hybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.
本研究纳入了1996年和1997年从土耳其54个不同省份采集的634份鸽粪样本中分离出的总共26株环境新生隐球菌新生变种菌株。脉冲场凝胶电泳（PFGE）结果显示，这26株菌株可分为24种不同的PFGE模式。在交配型研究中，26株菌株中，20株为MATα型，4株为MATa型，1株为MATa/α型，1株无法用STE20特异性引物分型。通过聚合酶链反应分型，所有分离株均为血清型A。这些分离株之间广泛的异质性表明，土耳其可能不存在单一的克隆群体。此外，AMATa/DMATα杂种的存在可能表明在土耳其环境中存在AMATa交配型的菌株。
