Riccia D N Della, Bizzini F, Perilli M G, Polimeni A, Trinchieri V, Amicosante G, Cifone M G
Department of Experimental Medicine, University of L'Aquila, L'Aquila, Italy.
Oral Dis. 2007 Jul;13(4):376-85. doi: 10.1111/j.1601-0825.2006.01291.x.
To analyze the anti-inflammatory effects of Lactobacillus brevis extracts on periodontitis patients and to investigate the involved mechanisms in vitro on activated macrophages.
Eight healthy subjects and 21 patients with chronic periodontitis were enrolled to analyze the effect of L. brevis-containing lozenges on periodontitis-associated symptoms and signs. Before and after the treatment, the patients received a complete periodontal examination. Saliva samples, collected before and after treatment, were analyzed for metalloproteinase and nitric oxide synthase (NOS) activity, immunoglobulin-A (IgA), prostaglandin E(2) (PGE(2)) and gamma-interferon (IFN-gamma) levels. Arginine deiminase (AD) and NOS activities were determined through a radiometric assay. Metalloproteinases were assayed by zymogram and Western blotting, whereas IgA, PGE(2) and IFN-gamma were assayed by enzyme-linked imunosorbent assay tests.
The treatment led to the total disappearance or amelioration of all analyzed clinical parameters in all patients. This was paralleled to a significant decrease of nitrite/nitrate, PGE(2), matrix metalloproteinase, and IFN-gamma levels in saliva samples.
Our results suggest that the anti-inflammatory effects of L. brevis could be attributed to the presence of AD which prevented nitric oxide generation. Our findings give further insights into the knowledge of the molecular basis of periodontitis and have a potential clinical significance, giving the experimental ground for a new innovative, simple and efficacious therapeutical approach of periodontal disease.
分析短双歧杆菌提取物对牙周炎患者的抗炎作用，并在体外研究其对活化巨噬细胞的作用机制。
招募8名健康受试者和21名慢性牙周炎患者，分析含短双歧杆菌含片对牙周炎相关症状和体征的影响。治疗前后，患者接受全面的牙周检查。对治疗前后采集的唾液样本进行分析，检测金属蛋白酶和一氧化氮合酶（NOS）活性、免疫球蛋白A（IgA）、前列腺素E2（PGE2）和γ干扰素（IFN-γ）水平。通过放射测定法测定精氨酸脱亚氨酶（AD）和NOS活性。通过酶谱法和蛋白质印迹法检测金属蛋白酶，而通过酶联免疫吸附测定法检测IgA、PGE2和IFN-γ。
治疗使所有患者所有分析的临床参数完全消失或改善。这与唾液样本中亚硝酸盐/硝酸盐、PGE2、基质金属蛋白酶和IFN-γ水平的显著降低相平行。
我们的结果表明，短双歧杆菌的抗炎作用可能归因于AD的存在，其可阻止一氧化氮的产生。我们的研究结果为牙周炎分子基础的认识提供了进一步的见解，具有潜在的临床意义，为牙周疾病一种新的创新、简单且有效的治疗方法提供了实验依据。
