Department of Life, Health & Environmental Sciences, University of L'Aquila, Building Rita Levi Montalcini, Coppito, 67100 L'Aquila, Italy.
Ph.D. School in Medicine and Public Health, University of L'Aquila, Building Rita Levi Montalcini, Coppito, 67100 L'Aquila, Italy.
Biomolecules. 2024 May 20;14(5):605. doi: 10.3390/biom14050605.
Enamel plays an essential role in protecting the underlying layers of the human tooth; therefore, preserving it is vital. This experimental study aimed to evaluate the potential ability of to counteract the action of a demineralizing agent on dental enamel morphology and mineral composition in vitro.
The sample consisted of 12 healthy human posterior teeth. The coronal portion of each tooth was subdivided into two equal parts longitudinally. The specimens were randomly divided into four groups: artificial saliva, suspension, demineralizing agent (DA), and DA plus . Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were used to evaluate the surface micromorphology and the mineral content, respectively. The statistical analysis was conducted using a one-way ANOVA, followed by Tukey's post hoc test.
SEM analysis did not highlight significant changes in the enamel microstructure of -treated specimens compared to the control. DA-induced damage to the enamel structure was drastically reduced when the specimens were contextually exposed to the probiotic. The treatment with DA substantially reduced the weight % of crucial enamel minerals, i.e., Ca and P. Notably, the probiotic was able to reverse the demineralization process, bringing Ca and P weight % back to basal levels, including the Ca/P ratio.
The findings indicate that is able to efficiently protect the dental enamel surface from the damage caused by DA and increase the enamel resistance to demineralization. Overall, confirms its efficacy in preventing or counteracting the action of carious lesions through a novel mechanism that protects the tooth surface under a chemical challenge that mimics the caries process.
牙釉质在保护人类牙齿的深层组织方面起着至关重要的作用；因此，保护它至关重要。本实验研究旨在评估 在体外对抗脱矿剂对牙釉质形态和矿物质组成的作用的潜在能力。
样本由 12 颗健康的人后牙组成。每个牙齿的冠部分别沿纵向分为两等份。标本随机分为四组：人工唾液、 混悬液、脱矿剂（DA）和 DA 加 。扫描电子显微镜（SEM）和能谱（EDS）分别用于评估表面微观形貌和矿物质含量。统计分析采用单因素方差分析，然后进行 Tukey 事后检验。
SEM 分析未显示 处理的标本与对照相比牙釉质微观结构有显著变化。当标本在上下文中暴露于益生菌时，DA 对牙釉质结构的损伤明显减少。DA 处理大大降低了关键牙釉质矿物质的重量%，即 Ca 和 P。值得注意的是，益生菌能够逆转脱矿过程，使 Ca 和 P 的重量%恢复到基础水平，包括 Ca/P 比。
研究结果表明， 能够有效地保护牙釉质表面免受 DA 造成的损害，并提高牙釉质对脱矿的抵抗力。总的来说， 证实了其通过一种新的机制预防或对抗龋齿病变的作用，该机制通过保护牙齿表面免受化学挑战的影响，从而模仿龋齿过程。
