Li Yin-ping, Qiao You-jie, Wu Zi-xia, Qian Fang-fang, Yao Yong-ming, Yu Yan, Wu Yao
Tianjin Tianhe Hospital, Tianjin 300050, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Jun;19(6):365-8.
To investigate effects of the integrated traditional Chinese medicine Xuebijing injection on thrombomodulin (TM) and endothelial cell protein C receptor (EPCR) in septic rats.
Ninety-six healthy Wistar rats were randomly divided into four groups: control group, sham operation group, cecal ligation and puncture (CLP) model group, and Xuebijing-treated group. Sepsis was reproduced by CLP. The two latter groups were divided into five subgroups of 2, 8, 24, 48 and 72-hour with 8 rats in each subgroup. Tissue samples from liver and lung were collected to determine tissue TM and EPCR mRNA expression.
TM and EPCR mRNA expressions were observed in liver and lung in control group and sham operation group, while with no significant differences at 2 hours post-CLP (both P>0.05). TM and EPCR gene expression levels in tissues were significantly increased to certain extent at 8-48 hours (all P<0.01), and were dramatically decreased following Xuebijing injection at 72 hours post-CLP (both P>0.05). Also, treatment with Xuebijing injection markedly decreased TM and EPCR mRNA levels to certain extents at 8 and 24 hours, and markedly increased at 48 and 72 hours compared with those of model group.
These data suggest that Xuebijing injection could raise TM and EPCR mRNA expression, thereby it might be effective in prevention of development of severe sepsis.
探讨中药复方制剂血必净注射液对脓毒症大鼠血栓调节蛋白（TM）及内皮细胞蛋白C受体（EPCR）的影响。
将96只健康Wistar大鼠随机分为四组：对照组、假手术组、盲肠结扎穿孔（CLP）模型组和血必净治疗组。采用CLP复制脓毒症模型。后两组再分为2、8、24、48和72小时五个亚组，每组8只大鼠。采集肝和肺组织样本，检测组织TM和EPCR mRNA表达。
对照组和假手术组肝和肺组织中均观察到TM和EPCR mRNA表达，CLP术后2小时无显著差异（均P>0.05）。组织中TM和EPCR基因表达水平在8 - 48小时有一定程度显著升高（均P<0.01），CLP术后72小时血必净注射后显著降低（均P>0.05）。此外，与模型组相比，血必净注射治疗在8和24小时能一定程度显著降低TM和EPCR mRNA水平，在48和72小时显著升高。
这些数据表明，血必净注射液可提高TM和EPCR mRNA表达，从而可能对预防严重脓毒症的发展有效。
