Ji Jun, Zhou Feihu, Yue Hui, Song Qing
Department of Critical Care Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
Exp Ther Med. 2014 Jun;7(6):1745-1751. doi: 10.3892/etm.2014.1639. Epub 2014 Mar 27.
This study aimed to investigate the protective mechanism of Xuebijing injection (XBJ) against heat stroke (HS) in rats. Adult male Sprague Dawley rats were randomly divided into normal control (NC), normal saline-treated HS (NS-HS) and XBJ-treated HS (XBJ-HS) groups. At 47 and 57 min from the initiation of heat stress (42.5-43.5°C), the plasma levels of certain cytokines [interleukin (IL)-1β, IL-6 and tumor necrosis factor-α], biochemical indicators (creatinine, blood urea nitrogen, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase) and coagulation indicators (activated partial thromboplastin time, prothrombin time, fibrinogen degradation products and D-dimer) were detected, and microscopy of the liver tissue of the rats was conducted. At 47 and 57 min after the initiation of the heat stress, the levels of the cytokines, coagulation indicators and biochemical indicators in the NS-HS group were significantly higher than those in the NC group (P<0.05). In the NS-HS group, the levels of the aforementioned indices were significantly reduced compared with those in the NC and NS-HS groups (P<0.05). In the NS-HS group, serious liver cell congestion, nuclear swelling and central vein dilation were visible, along with the appearance of bubbles in the liver tissue. In the XBJ-HS group, only a small number of congestive liver cells were identified, with occasional nuclear swelling but no bubbles, which was similar to the observations in the NC group. Early intervention treatment of HS with XBJ is able to reduce the systemic inflammatory response and coagulation activity and decrease the tissue ischemia and injury degree, thus extending the survival time of rats with HS.
本研究旨在探讨血必净注射液（XBJ）对大鼠热射病（HS）的保护机制。成年雄性Sprague Dawley大鼠被随机分为正常对照组（NC）、生理盐水处理的热射病组（NS-HS）和血必净注射液处理的热射病组（XBJ-HS）。在热应激（42.5 - 43.5°C）开始后的47分钟和57分钟，检测某些细胞因子[白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α]、生化指标（肌酐、血尿素氮、天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶）和凝血指标（活化部分凝血活酶时间、凝血酶原时间、纤维蛋白原降解产物和D-二聚体）的血浆水平，并对大鼠肝脏组织进行显微镜检查。在热应激开始后的47分钟和57分钟，NS-HS组的细胞因子、凝血指标和生化指标水平显著高于NC组（P<0.05）。与NC组和NS-HS组相比，NS-HS组上述指标水平显著降低（P<0.05）。在NS-HS组中，可见严重的肝细胞充血、核肿胀和中央静脉扩张，同时肝脏组织中出现气泡。在XBJ-HS组中，仅发现少量充血肝细胞，偶尔有核肿胀但无气泡，这与NC组的观察结果相似。血必净注射液对热射病进行早期干预治疗能够降低全身炎症反应和凝血活性，减轻组织缺血和损伤程度，从而延长热射病大鼠的存活时间。
