Leonardi Fabiana, Attorri Lucilla, Benedetto Rita Di, Biase Antonella Di, Sanchez Massimo, Tregno Francesca Pellizzari, Nardini Mirella, Salvati Serafina
Department of Food Science, Nutrition and Health, Istituto Superiore di Sanità, V.Ie Regina Elena, Roma, Italy.
Free Radic Res. 2007 Jul;41(7):748-56. doi: 10.1080/10715760701324067.
In view of the promising use of n-3 polyunsaturated fatty acids (PUFAs) in the prevention and treatment of neurological diseases, it is necessary to ascertain the lack of detrimental oxidative effects. We evaluated short- and long-term effects of 25, 50 and 75 muM docosahexaenoic acid (DHA) supplementation on the oxidative status of C6 glial cells. DHA was incorporated into cells dose and time dependently without any cytotoxic effect. Reactive oxygen species (ROS) level was related to DHA dose and supplementation time. At the lowest dose no significant increase in ROS values was observed at hour 24. Low doses of DHA strengthened the cellular antioxidant defence system as highlighted by a raise in both GPX and catalase activity, and the decreased levels of lipid peroxidation. This effect was pronounced at 24 h of supplementation, almost disappeared at hour 48, while after 72 h an opposite effect was observed: lipid peroxidation increased concomitantly with DHA doses. Therefore, the final effect of DHA on cellular redox status is dependent on dose and time supplementation.
鉴于n-3多不饱和脂肪酸（PUFAs）在预防和治疗神经疾病方面具有广阔的应用前景，有必要确定其不存在有害的氧化作用。我们评估了补充25、50和75μM二十二碳六烯酸（DHA）对C6神经胶质细胞氧化状态的短期和长期影响。DHA以剂量和时间依赖性方式被细胞摄取，且无任何细胞毒性作用。活性氧（ROS）水平与DHA剂量和补充时间有关。在最低剂量下，24小时时未观察到ROS值有显著增加。低剂量的DHA增强了细胞抗氧化防御系统，这表现为谷胱甘肽过氧化物酶（GPX）和过氧化氢酶活性的提高以及脂质过氧化水平的降低。这种效应在补充24小时时明显，48小时时几乎消失，而72小时后观察到相反的效应：脂质过氧化随DHA剂量增加而增加。因此，DHA对细胞氧化还原状态的最终影响取决于剂量和补充时间。
