Fjaertoft Gustav, Håkansson Lena Douhan, Pauksens Karlis, Sisask Gregor, Venge Per
Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
Scand J Infect Dis. 2007;39(6-7):525-35. doi: 10.1080/00365540601113693.
Neutrophil CD64 expression is a diagnostic marker for the early detection of bacterial infections. The aim was to investigate the kinetics of neutrophil CD64 expression during bacterial infection and the possible impact of surgical trauma. Blood samples were collected daily during 3 d after admission for analysis by flow cytometry of the surface expressions on neutrophils and monocytes of CD64, CD16, CD32, CD11b/CD18 and CD35, and analysis of serum CRP and blood WBC. Serum concentrations of IFNgamma, G-CSF, IL-6 and IL-8 were also analysed in adults. Eight children and 19 adult patients with bacterial infections, 12 patients admitted for hip-arthroplasty because of coxarthrosis and 30 healthy adults were studied. Neutrophil CD64 was increased all 3 d after start of treatment (p<0.0001) in children and adults with bacterial infections. The postoperative increase after surgery was less than the increase seen during bacterial infections (p<0.0001). CRP, G-CSF, IL-6 and IL-8 were raised both in bacterial infections and after surgery. Our results indicate that the expression of CD64 on neutrophils is a specific sign of bacterial infections. Neutrophil expression of CD64, therefore, seems to be a promising tool for the early detection of bacterial infections even during surgery.
中性粒细胞CD64表达是早期检测细菌感染的诊断标志物。目的是研究细菌感染期间中性粒细胞CD64表达的动力学变化以及手术创伤可能产生的影响。入院后3天内每天采集血样，通过流式细胞术分析中性粒细胞和单核细胞表面CD64、CD16、CD32、CD11b/CD18和CD35的表达，并分析血清CRP和血液白细胞。还对成人血清中IFNγ、G-CSF、IL-6和IL-8的浓度进行了分析。研究了8名患有细菌感染的儿童和19名成年患者、12名因髋关节病接受髋关节置换术的患者以及30名健康成年人。患有细菌感染的儿童和成人在开始治疗后的3天内中性粒细胞CD64均升高（p<0.0001）。术后升高幅度小于细菌感染期间的升高幅度（p<0.0001）。CRP、G-CSF、IL-6和IL-8在细菌感染和手术后均升高。我们的结果表明，中性粒细胞上CD64的表达是细菌感染的特异性标志。因此，中性粒细胞CD64表达似乎是即使在手术期间早期检测细菌感染的一个有前景的工具。
